戊糖片球菌P36对HepG2细胞抗氧化能力影响的初步研究

Preliminary study on the effect of Pediococcus pentosaceus P36 on the antioxidant capacity of HepG2 cells

目的:研究戊糖片球菌P36对Hep G2细胞抗氧化能力的影响。方法:将培养的Hep G2细胞分为3组:空白对照组(不加H2O2)、模型组(加入H2O2)和乳酸菌组(加入戊糖片球菌P36和H2O2)。细胞培养至12 h和24 h时分别测定其上清液和细胞裂解液的抗氧化活性。利用4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole,DAPI)对Hep G2细胞进行染色,在荧光显微镜下观察不同组细胞核形态的变化。结果:添加戊糖片球菌P36 12 h后,与模型组相比,细胞上清液中的总抗氧化力(total antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxide dismutase,SOD)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和过氧化氢酶(catalase,CAT)活力均显著提高(p<0.05)。细胞裂解液中的超氧化物歧化酶(superoxide dismutase,SOD)活力也显著提高(p<0.05);与模型组相比,添加P36 24 h后,细胞上清液中的抗氧化酶活力均显著增加(p<0.05),还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)的含量分别显著降低(p<0.05),细胞裂解液中的SOD活力显著降低了19.76%(p<0.05)。加入戊糖片球菌P36 24 h后,与模型组相比,Hep G2细胞受损程度降低,大部分细胞状态趋于正常。结论:戊糖片球菌P36可以提高氧化应激状态下Hep G2细胞的抗氧化能力。

Objective： The aim of the present study is to investigate the effect of Pediococcus pentosaceus P36 on antioxidant function of Hep G2 cells under oxidative stress. Method： Hep G2 cells were randomly divided into 3 groups： Control,model（ H2 O2 induced oxidative stress） and treatment（ H2 O2 plus Pediococcus pentosaceus P36） groups. Antioxidant activity in culture supernatants and lysates of Hep G2 cells at 12 h and 24 h were measured.4＇,6-Diamidino-2-phenylindole（ DAPI） staining was applied to observe cell morphology in different groups by fluorescence microscopy. Results： Total antioxidant capacity（ T-AOC）,superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-Px） and catalase（ CAT） activities in the culture supernatant,as well as SOD activity in the cell lysate at 12 h in the treatment group were significantly increased when compared with their counterparts in the model group（ p〈0.05）.At 24 h,the activities of antioxidant enzymes（ p〈0.05） were significantly increased（ p〈0.05）,and the content of glutathione（ GSH） and malondialdehyde（ MDA） in the culture supernatant in the treatment group were significantly decreased（ p〈0.05）.A significant decrease of 19.76% in SOD activity in the cell lysate was observed for the treatment group compared with the model group（ p〈0.05）. Moreover,at 24 h,the level of damaged cells was reduced in the treatment group than that in the model group,and most cells in the former group had normal morphology.Conclusion： The addition of Pediococcus pentosaceus P36 could increase antioxidant function of Hep G2 cells under oxidative stress.