苏氨酸铁对缺铁性贫血大鼠铁营养状况的改善

Effects of ferrous threonine chelate on improving the iron status of iron-deficiency anemia rats

目的:探讨苏氨酸铁(Thr-Fe)对缺铁性贫血(IDA)大鼠铁营养状况的改善作用。方法:以低铁饲料饲喂雄性初断乳斯泼累格·多雷(SD)大鼠建立IDA模型,设置3个不同Thr-Fe剂量组和阳性对照组、低铁对照组、空白对照组,每组6只,每天灌胃1次,连续给药21 d,Thr-Fe剂量组分别给予8、16、32 mg/kg·bw的Thr-Fe,阳性对照组给予32 mg/kg·bw硫酸亚铁。结果:较低铁对照组,Thr-Fe高剂量组大鼠体重及血红蛋白、血清铁蛋白等血液指标显著提高(p<0.01),血清转铁蛋白(p<0.01)和总铁结合力(p<0.05)显著降低,各Thr-Fe剂量组大鼠游离原卟啉显著降低(p<0.01)。Thr-Fe高剂量组IDA大鼠各项指标改善优于阳性对照组。结论:低铁膳食补充Thr-Fe可以改善IDA大鼠的铁营养状况,提高功能铁及贮存铁含量,促进机体铁转运。Thr-Fe对IDA大鼠体重及血红蛋白等的影响呈剂量相关性,Thr-Fe对IDA大鼠铁营养状况的改善效果优于硫酸亚铁。

Objective： The effects of ferrous threonine chelate（ Thr-Fe） on improving the iron status of rats suffering iron deficiency anemia（ IDA） were investigated.Method： The IDA model of male weaning SD rats was established by iron-deficiency diet.And IDA rats were randomly divided into three Thr-Fe dose groups,positive control group,low-Fe control group,basing on their hemoglobin contents and body weights.A blank control group was set up,in addition.There were 6 rats in each group.The 8,16,32 mg/kg·bw of Thr-Fe were orally administered to the three Thr-Fe dose groups once a day,respectively,lasting21 days uninterruptedly; 32 mg/kg·bw of ferrous sulfate was given to positive control group in the same way.Results： After recovery test,the blood indexes such as hemoglobin and serum ferritin of IDA rats and the body weights in Thr-Fe high dose groups were improved and significantly higher（ p〈0.01） than those in low-Fe control group,while the transferrin（ p〈0.01） and total iron binding capacity（ p〈0.05） were significantly lower,and the free erythrocyte protephyrin of the three Thr-Fe dose groups were significantly lower（ p〈0.01）. The indexes of IDA rats in high dose group of Thr-Fe were better than that in positive control group.Conclusion： Supplements of Thr-Fe could improve the iron status of IDA rats,increasing the contents of functional iron and iron storage,promoting iron transportation. Effects of Thr-Fe on body weights and hemoglobin of IDA rats are dose-dependent and the effects of Thr-Fe on iron status of IDA rats are better than that of ferrous sulfate.