眼内前后房压强差在体连续测量的实验研究

Experimental study on the in vivo continuous measurement of pressure difference between the anterior and the posterior chambers

为了探讨兔眼前节内前后房压强差随时间变化的规律,设计了一套前后房压强差在体测量装置。利用连通器原理,搭建在体测量眼内前后房压强差的平台。通过注射复方卡波姆升高眼压的方法构建高眼压兔眼模型,实现正常和高眼压兔眼的前后房压强差的在体24小时连续测量。设计的本装置对于眼内微小的压强差会产生比较敏感的反应。正常兔眼前后房压强差随时间变化,一天的变化范围为5.84~96.84 Pa,反映出生理性规律的存在。高眼压性兔眼多呈现前房压强高于后房压强的状况,符合自适应性调整学说。本文所设计的方法和装置可以较好地实现眼内前后房压强差的测量,且前后房压强差在一天当中随时间呈规律性变化。

A set of device for the in vivo measurement of the pressure difference between the anterior and the posterior chambers（PDAP） was designed to investigate the temporal varying rules of PDAP in the anterior segment of rabbit eyes. A platform was established for the in vivo measurement of PDPA according to the mechanism of joint implement. Rabbit models with high intraocular pressure（IOP） were constructed by means of injecting Carbomer into anterior chamber to increase IOP. The in vivo 24 hours continuous measurements of PDAP were performed for normal rabbit eye and eye with high IOP. The developed device could sensitively response to the small pressure difference in eye. The pressure difference in the normal rabbit eye varied with time, and the variation range during a whole day was5.84–96.84 Pa which reflected the existence of physiological rule. For the rabbit eye with high IOP, pressure in anterior chamber was higher than that in posterior chamber which was in consistence with the theory of self-adaptation adjustment. The present study indicates that the approaches and device designed in this paper can well implement the measurement of PDAP as well as the temporal varying rules of PDAP in the anterior segment during a whole day.