网眼状多孔陶瓷支架的冷冻干燥制备及其生物性能

Preparation and Biological Properties of Reticulated Porous Alumina Bio-ceramic Scaffold by Freezing-casting

采用叔丁醇溶液为冷冻媒介,冷冻干燥技术制备出网眼状多孔氧化铝陶瓷支架。通过改变冷冻温度影响网眼孔陶瓷支架孔径,并对不同孔径的网眼孔支架进行生物性能分析。结果表明,采用叔丁醇冷冻干燥技术制备的网眼孔支架主要以开孔为主,网眼孔支架孔径随着冷冻温度的降低而减少,但支架的开孔孔隙率不受冷冻温度影响。体外模拟实验表明,大孔径的网眼孔支架有利于细胞在表面生长,细胞贴壁生长,形态正常。支架表面细胞的附着率随支架孔径的增大而增多。

Using crystallized TBA （Tert,-butyl alcohol） solution as template, reticulated macrospores of Al2O3 bio-ceramic scaffolds were fabricated by the freeze casting. The influence of the pore size of the reticulated macrospores by changing the freezing temperature was investigated. The morphology and biological properties of the reticulated macrospores scaffold with different pore size were investigated. The results show that using crystallized TBA solution as template, the reticulated macrospores showing open porosity are fabricated by the freeze casting, the pore size of the reticulated macrospores is decreased with the freezing temperature lowered. The freezing temperature does not influence its open porosity. Vitro cellular assay indicates that Al2O3 bio-ceramic scaffolds support the structure of reticulated macrospores with larger size is more conducive to cell growth, cell surface structure clear, spreading in good shape. With the increasing of the pore size of the reticulated pore, the adhesion rate on the scaffolds is gradually increased.