干扰表皮生长因子受体磷酸化对乳腺癌裸鼠移植瘤生长的抑制作用

Inhibitory effect of interfering the phosphorylation of epithelial growth factor receptor on breast cancer cell xenografts in nude mice

目的探讨干扰表皮生长因子受体（EGFR）的磷酸化过程作为乳腺癌靶向治疗的可能性和有效性。方法构建乳腺癌MDA-MB-231细胞的裸鼠移植瘤模型，通过腺病毒介导hSulf-1基因的表达，抑制EGFR的磷酸化，检测其对裸鼠移植瘤治疗的疗效。结果重组腺病毒Ad5-hSulfl介导的hsulf-1表达能产生明显的抗肿瘤效果，治疗开始后第21天，Ad5-hSulfl组肿瘤体积（670．83±65．02）mm^3，而空白对照组为（1815．33±214．43）mm^3，差异有统计学意义（P=0．000）。取瘤体组织检测，发现hSulf-1的表达没有影响EGFR的总体表达含量，但p-EGFR水平明显下降。结论hSulf-1表达能明显抑制EGFR的磷酸化过程，从而产生明显的肿瘤抑制效应。

Objective To investigate the effect of interfering phosphorylation of epithelial growth factor receptor （EGFR） on breast cancer cell xenografts in nude mice. Methods Breast cancer cell MDA- MB -231 xenograft model was established in nude mice and treated with adenovirus Ad5 -hSulfl. The effect of hSulf - 1 gene was examined on the inhibition of EGFR phosphorylation and xenograft growth. Results The recombinant adenovirus AdS - hSulfl expressed hSulf- 1 factor and inhibited tumor growth. There was an obvious difference in tumor volume between Ad5 - hSulfl group （ 670. 83 ± 65.02 ）mm3 and blank control group （1 815.33±214.43） mm3 （P = 0. 000）. The expression of hSulf- 1 did not affect total EGFR expression but decreased p - EGFR expression level. Conclusion The expression of hSulf - 1 can inhibit the EGFR phosphorylation and then inhibit the breast cancer xenograft growth.