Toll样受体2在结肠癌组织的表达及其对结肠癌细胞生长的影响

Expression of Toll - like receptor 2 in colon tumor and its effects on cell growth

目的观察Toll样受体2（TLR2）在结肠癌中的表达，探讨TLR2信号对结肠癌细胞生长的影响。方法免疫组织化学染色法检测20例结肠癌组织及配对癌旁组织中TLR2蛋白表达；应用TLR2配体Pam3Cys（P3C）激活结肠癌细胞株SW480和HCT116的TLR2信号，通过细胞计数试剂盒（CCK-8）实验、划痕实验、Transwell实验观察其对结肠癌细胞增殖、迁移、侵袭的影响；Western blot实验检测TLR2信号激活后结肠癌细胞磷酸肌醇3激酶（PI3K）/蛋白激酶B（Akt）和核因子-κB（NF-κB）蛋白表达。结果结肠癌组织和配对癌旁组织中TLR2蛋白表达阳性率分别为85.0%（17/20）和40.0%（8/20），两者免疫组织化学评分差异有统计学意义（χ2＝8.640，P＝0.003）；TLR2可以促进结肠癌细胞增殖（SW480组：1.496±0.041比1.003±0.002，t＝20.802，P＝0.000；HCT116组：1.859±0.074比1.002±0.003，t＝20.430，P＝0.000），同时可以促进结肠癌细胞的迁移能力[SW480组：（72.00±11.78）%比（45.67±6.13）%，t＝3.434，P＝0.026；HCT116组：（64.33±15.52）%比（32.33±11.32）%，t＝2.885，P＝0.045]和侵袭能力（SW480组：81.3±5.3比31.3±3.7，t＝13.398，P＝0.000；HCT116组：58.0±5.7比19.7±3.3，t＝10.072，P＝0.001）。TLR2信号能够激活结肠癌细胞PI3K/Akt和NF-κ通路。结论TLR2在结肠癌中高表达，能够通过PI3K/Akt和NF-κB通路促进结肠癌细胞增殖、迁移、侵袭。

Objective To observe the expression of Toll-like receptor-2 （TLR2） in colon tumor and its effects on cell growth by activating TLR2 signaling.Methods Immunohistochemistry was used to detect the protein expression of TLR2 in twenty paired colon cancer patient tissue samples and normal adjacent tissue samples. Stimulated with TLR2 ligand Pam3Cys （P3C）, cell counting kit-8 （CCK-8）, wound healing and Transwell assays were used to observe effects on proliferation, migration and invasion of colon tumor cell line SW480 and HCT116. Western blotting was used to measure protein expression of phosphatidylinositol 3 kinase （PI3K）/protein kinase B （Akt） and nuclear factor-κB （NF-κB） signaling pathways with P3C treatments.Results Positive rate of TLR2 expression in colon tumor tissues is 85.0% （17/20）, while that in adjacent normal tissues is 40.0% （8/20）, and the expression of TLR2 in the colon tumors was shown to be significantly high compared with adjacent normal tissue using immunohistochemistry （χ2=8.640, P=0.003）. TLR2 can promote the proliferation of colon cancer cells （SW480 group： 1.496±0.041 vs. 1.003±0.002, t=20.802, P=0.000; HCT11 group： 1.859±0.074 vs. 1.002±0.003, t=20.430, P=0.000）, and also promote the migration and invasion of colon tumor cells [SW480 group： （72.00±11.78）% vs. （45.67±6.13）%, t=3.434, P=0.026; HCT116 group： （64.33±15.52）% vs. （32.33±11.32）%, t=2.885, P=0.045） （SW480 group： 81.3±5.3 vs. 31.3±3.7, t=13.398, P=0.000; HCT116 group： 58.0±5.7 vs. 19.7±3.3, t=10.072, P=0.001）. PI3K/Akt and NF-κB signaling pathways were up-regulated in TLR2-driven colon tumor cells.Conclusion TLR2 is highly expressed in colon tumor, and promotes proliferation, migration and invasion of colon tumor cells via PI3K/Akt and NF-κB signaling pathways.