基于电子束熔融技术制备的多孔钛合金的细胞相容性

The study of cytocompatibility of electron beam melting fabricated porous ti6al4v scaffolds

目的探讨基于电子束熔融（EBM）技术制备的多孔钛合金的细胞相容性。方法采用EBM技术制备两种孔径规格：小径组（EBMS，640μm）和大孔径组（EBML，1200Izm）；对孔径、孔隙率、单位体积比表面积、渗透率、表面形貌以及蛋白吸附水平进行检测；将人骨髓来源间充质干细胞接种于多孔钛合金培养，对细胞黏附与形态、细胞增殖活性以及成骨分化水平进行评估。结果th-fL径组EBMS具有较低的渗透率[EBMS：（1．03±0．03）×10^-9m^2，EBML：（1．64±0．07）×10^-9m^2]，较高的单位体积比表面积[EBMS：（2．41±0．04）／mm，EBML：（1．69±0．10）／mm]和蛋白吸附水平[EBMS5：（1±4）μg，EBML：（25±3）μg]。两种多孔钛合金都支持间充质干细胞的黏附、增殖和成骨分化。EBMS因为较大的单位体积比表面积具有较高的蛋白吸附水平，所以表现出更好的细胞相容性。结论基于EBM技术制备的多孔钛合金具有良好的细胞相容性；不同的孔隙结构会对多孔钛合金的细胞相容性产生影响。

Objective The aim of the present study was to evaluate the cytocompatibility of Electron beam melting （EBM） fabricated porous Ti6A14V. Methods The porous Ti6A14V were fabricated using the EBM process with either a small pore size （EBMS, 640 μm） or a large one （EBML, 1 200 μm）. The two types of samples were characterized in respect of microstructure, porosity, specific surface area, permeability, surface morphology and protein adsorption. The human bone marrow derived mesenchymal stem cells （hBMSCs） were seeded and cultured on the samples. The cell attachment and morphology, pro- liferation activity and osteogenic differentiation were evaluated subsequently. Results The EBMS samples showed lower permeability [EBMS： （1.03 ±0.03） × 10^9 m^, EBML： （1.64 ±0.07） × 10^9 m2], higher specific surface area [ EBMS： （2.41 ±0. 04）/m, EBML： （ 1.69 ±0. 10）/m] and more protein adsorption [EBMS： （51±4） μg, EBML： （25±3） μg]. Both samples EBMS and EBML could support the attachment, proliferation and differentiation of hBMSCs. Nevertheless, the EBMS scaffolds were rela- tively more compatible with hBMSCs than the EBML probably for their larger specific surface area and more protein adsorption. Conclusion The EBM fabricated porous Ti6A14V scaffolds possesses appropriate cyto- compatibility. The difference in pore structure could impact somehow the cytoeompatibility of the scaffolds.