肥胖小鼠袖状胃切除术模型的建立

Establishment of sleeve gastrectomy model in obese mice

目的建立高效、成功率高的肥胖小鼠袖状胃切除术（sG）模型。方法选取6周龄健康的无特定病原体（SPF）级雄性C57BL／6J鼠40只，体重为（21．05±1．07）g。小鼠随机分为两组：对照组（Control）8只，采取普通饲料喂养；高脂组32只，高脂饲料喂养16周，诱导肥胖小鼠模型。16周以后将体重38g以上的肥胖小鼠入组，并随机分为sG组（16只）和假手术组（Sham8只），其中SG组和假手术组平均体重分别为（47．31±2．35）、（45．38±2．97）g。术后检测小鼠体重、空腹血糖和空腹胰岛素水平，并行口服葡萄糖耐量试验（OGTT）检测其胰岛素敏感性改变。结果假手术组小鼠体重术后第6天开始回升，术后8周左右体重已超越术前水平；SG组小鼠术后早期体重进行性下降，且术后8周仍明显低于假手术组[（36．50±2．31）g比（46．20±2．05）g；P=0．001]。sG组术后8周空腹血糖较假手术组和高脂组显著降低（P=0．001、0．001），分别为（5．38±1．07）、（7．84±0．80）、（8．62±1．31）mmol／L。SG组术后8周空腹胰岛素较假手术组和高脂组显著降低（P=0．001、0．001），分别为（205．75±42．80）、（293．50±30．78）、（327．88±46．23）mU／L。同时，口服葡萄糖耐量试验（OGTT）和胰岛素抵抗指数表明，sG可显著改善肥胖小鼠胰岛素敏感性（P=0．015、0．003）。结论为sG治疗肥胖和糖尿病机制的研究提供一个更为合理、有效的模型。

Objective 2Fo establish a sleeve gastrectomy （SG） model in obese mice with high efficiency and high success rate. Methods Forty 6 - week - old male C57BL/6J mice weighing （21.05 ± 1.07） g were randomized into two groups ： control group （ mice were fed on regular chow diet, n = 8） and high-fat diet group （mice were fed on high fat diet for 16 weeks to induce obesity, n= 32）. After 16 weeks, obese mice over 38 g were randomized into SG group （n = 16） and sham operation group （n = 8）. The average body weight in SG group and sham group was （47.31±2. 35） and （45.38±2.97） g respectively. The body weight, fasting blood glucose, and fasting insulin levels were measured at 8th week postoperation. The oral glucose tolerance test （OGTT） was used to evalue the insulin sensitivity. Results The body weight in the sham operation group began to rebound from the 6th day after surgery and was higher than the preoperative level at 8th week after surgery. The body weight in the SG group was de- creased early after surgery, and was still significantly lower than the sham group after 8 weeks [ （36. 50 ± 2. 31 ） g vs. （46. 20±2.05） g; P =0. 0011. The fasting plasma glucose in the SG group was significantly lower than that in the sham group and the high - fat group （ P = 0. 001, 0. 001 ） after 8 weeks postopera- tively, and fasting plasma glucose was （ 5.38 ± 1.07 ） , （ 7.84± 0. 80 ） and （ 8.62± 1.31 ） mmol/L respectively. Fasting insulin in the SG group was significantly lower than that in the sham group and the high-fat group at 8th week after operation （P= 0.001, 0.001）, which was （205.75± 42. 80）, （293.50 ±30. 78）, （327. 88 ±46. 23 ） mU/L. The results of OGTY and the insulin resistance index showed that SG could significantly improve insulin sensitivity in obese mice （P = 0. 015, 0. 003）. Conclusion This research will provide us a more reasonable and effective model for the study of the treatment mechanisms of SG.