近日钟基因Clock单核苷酸多态性与心肌缺血的相关性

The association of circadian clock gene single nucleotide polymorphism with myocardial infarction

目的探讨近日钟基因Clock单核苷酸多态性与心肌缺血的关系。方法收集临床确诊的心肌缺血患者120例为心肌缺血组;同时收集临床体检健康人群78例为健康对照组。采用普通PCR、凝胶电泳、单碱基引物延伸法基因分型(SnapShot)等方法检测Clock基因单核苷酸多态性位点(rs3840267,rs3749474和rs1402963)在心肌缺血组和健康对照组中的基因型及等位基因频率分布,并分析其与心肌缺血的关系。结果 Clock基因rs3840267位点的基因型及等位基因频率分布在心肌缺血组和健康对照组中差异有统计学意义(P<0.05);而rs3749474和rs1402963位点基因型及等位基因频率分布在心肌缺血组和健康对照组比较差异无统计学意义(P>0.05)。结论 Clock基因rs3840267位点多态性与心肌缺血存在关联。

Objective To analyze the association of the circadian clock gene （Clock） single nucleotide polymorphism （SNP） with myocardial infarction. Methods 120 patients with myocardial infarction were served as myocardial infarction group, and 78 healthy cases were served as healthy control group. The polymerase chain reaction （PCR）, gel eleetrophoresis and SNaPshot were applied to detect the genotype of Clock. Chi-square test was used to test the genotypic and allelic frequency of Clock gene, and analyzed its association with myocardial infarction in Chinese Han population. Results There was statistical significance in distribution of genotypic and allelie frequency of rs3840267 between myocar dial infarction group and healthy control group （P〈0.05）. There was no statistical significance in distribution of geno- typic and allelie frequency of rs3749474 and rs1402963 between myocardial infarction group and healthy control group （P 〉0.05）. Conclusion The rs3840267 polymorphism in Clock gene was associated with myocardial infarction.