刺络泻血对非酒精性脂肪肝模型大鼠瘦素及脂联素的影响

Effects of Pricking Blood Therapy on Serum Level of Leptin and Adiponectin in Rat Model with Non-alcoholic Fatty Liver Disease

目的:观察刺络泻血干预对非酒精性脂肪肝模型大鼠血清中瘦素及脂联素的影响。方法:选取清洁级雄性SD大鼠40只,随机分为正常组(10只),模型组(10只),易善复组(10只),刺血干预组(10只)。选用高脂饮食饲料喂养法建立非酒精性脂肪肝模型。造模8周后,各造模组仍给予高脂饲料饲养,易善复组给予易善复0.159 g/(kg·d)灌胃,刺血干预组予"丰隆""太冲"穴三棱针刺血,治疗4周后取材,观察4组大鼠肝指数及肝脏病理变化,用酶联免疫吸附试验(ELISA)检测血清瘦素、脂联素的水平。结果:与正常组比较,模型组血清瘦素水平显著升高,脂联素水平显著降低,差异有统计学意义(P<0.05);与模型组比较,易善复组及刺血干预组瘦素水平明显下降,血清脂联素水平明显升高,差异有统计学意义(P<0.05);与易善复组比较,刺血干预组瘦素水平稍高,血清脂联素水平则稍低,差异无统计学意义(P>0.05)。结论:刺血干预可显著减轻非酒精性脂肪肝大鼠模型肝损伤,其机制可能与降低血清中瘦素水平,上调脂联素水平有关。

Objective： To investigate the effects of pricking blood therapy on serum level of leptin and adiponectin in non-alcoholic fatty liver model rats,and to explore the mechanism of pricking blood intervention on non-alcoholic fatty liver disease. Methods：Forty male SD rats were randomly divided into normal group（ n = 10）,model group（ n = 10）,essentiale group（ n = 10） and pricking blood group（ n = 10）. The non-alcoholic fatty liver model was established by high fat diet feed. After 8 weeks of modeling,the model groups were still fed with high-fat diet. Essentiale group was treated with Polyene Phosphatidylcholine Capsules0. 159 g/（ kg·d）,and the pricking blood group was treated with pricking blood therapy on Fenglong（ ST40） and Taichong（ LR3）. The liver index and liver pathological changes were observed after 4 weeks of treatment. The levels of serum leptin and adiponectin were measured by ELISA. Results： Compared with the normal group,the serum leptin level in the model group was significantly increased,and the content of adiponectin was significantly decreased. And the difference was statistically significant（ P 〈 0. 05）; Compared with the model group,the serum leptin level in the essentiale group and the pricking blood group was significantly decreased,and the content of adiponectin was significantly increased（ P 〈 0. 05）. The difference was statistically significant（ P 〈 0. 05）; Compared with Essentiale group,the leptin level was slightly higher in the pricking blood group,and the adiponectin content was slightly lower. The difference was not statistically significant（ P〉 0. 05）. Conclusion： This study shows that pricking blood therapy can significantly reduce liver injury in rats with non-alcoholic fatty liver disease. The mechanism may be related to down-regulating the serum level of leptin and up-regulating the adiponectin.