AIF-1基因多态性、体质指数与口腔癌发病风险的关联研究

Association of single nucleotide polymorphism of AIF-1 gene and body mass index with susceptibility to oral cancer

目的探讨同种异体移植炎症因子-1(allograft inflammatory factor-1,AIF-1)基因多态性、体质指数(body mass index,BMI)及其交互作用与口腔癌易感性的关系。方法采用病例对照研究,收集2010年9月~2016年12月经病理确诊的新发口腔癌患者300例及同期该医院体检人群445例。应用TaqMan探针技术检测AIF-1基因的单核苷酸多态性(single nucleotide polymorphisms,SNPs)。采用非条件Logistic回归分析计算比值比(odd ratios,OR)值及95%可信区间(confidence intervals,CI),分析AIF-1基因多态性、BMI及其交互作用与口腔癌易感性的关系。结果 GG基因型在共显性模型(OR=0.522,95%CI:0.319~0.855)和隐性模型(OR=0.573,95%CI:0.368~0.893)中均可降低口腔癌的发病风险。进一步的分层分析表明GG基因型在男性、BMI≥24.0kg/m^2、吸烟、非饮酒亚组中同样可降低口腔癌的发病风险。此外,结果还显示BMI<18.5 kg/m^2可增大口腔癌的发病风险,而BMI≥24.0 kg/m^2则是口腔癌的保护因素,并且AIF-1基因与BMI之间存在相乘交互作用。结论AIF-1基因rs2857595基因SNPs和BMI≥24.0 kg/m^2能降低口腔癌的发病风险,且rs2857595与BMI存在相乘交互作用。

Objective To explore the association of allograft inflammatory factor-1 （AIF-1） gene polymorphisms, body mass index （ BMI ） and their interactions with susceptibility to oral cancer. Methods A case-control study including 300 patients with pathologically confirmed oral cancer from September 2010 to December 2016 and 445 cancer-free controls was performed. The genotypes were determined by TaqMan genotyping assay. Unconditional Logistic regression was used to calculate odds ratios （OR） and 95% confidence intervals （CI） to assess the association of AIF-1 gene polymorphisms, BMI and their interactions with susceptibility to oral cancer. Results In the co-dominant genetic model （ OR = 0. 522, 95% CI： 0. 319-0. 855） and the recesswe genetic model （ OR = 0. 573, 95% CI：0. 368-0. 893） , subjects carrying GG genotype of rs2857595 had significantly reduced risk of oral cancer, respectively. Moreover, GG genotype was a clear pro- tective factor in male. individuals with BMI ≥24. 0 kg/m2, and smoking and non-drinking subgroups. By contrast, BMI 〈 18.5 kg/m2 was a risk factor of oral cancer, and BMI≥24.0 kg/m2 reduced the risk of oral cancer. In addition, statistical analysis suggested an interaction between AIF-1 rs2857595 polymorphism and BMI. Conclusions This study suggests that AIF-1 rs2857595 polymorphism and BMI≥24. 0 kg/m2 may be protective factors for oral cancer. And there may be a multi- plicative interaction between AIF-1 and BMI.