凝胶净化结合高效液相色谱-蒸发光散射检测器测定食用油脂中胆固醇含量

Determination of cholesterol content in edible oils and fats by gel permeation chromatography combined with high performance liquid chromatography-evaporative light scattering detector

建立了凝胶净化系统与高效液相色谱-蒸发光散射检测器结合对食用油脂中胆固醇含量测定的方法。结果表明:胆固醇质量浓度在2~200μg/m L范围内,胆固醇质量浓度的对数与峰面积对数线性关系良好,相关系数为0.998 9,检出限为10 mg/kg,回收率为94.01%~103.80%,相对标准偏差为1.12%~7.25%。该方法可有效减少烦琐的前处理过程对样品中胆固醇含量造成的损失,避免检测过程中出现溶剂响应和溶剂峰干扰,方法简单、可行、有效。

A method for the determination of cholesterol content in edible oils and fats was established by gel permeation chromatography system combined with liquid chromatography -evaporative light scattering detector. The results showed that the linearity correlation was good in the range of 2 -200 g/mL with correlation efficient 0.998 9. The limit of detection, recovery rate and relative standard deviation were 10 mg/kg, 94.01% - 103.80% and 1.12% -7.25% respectively. The method could effectively reduce the loss of cholesterol caused by cumbersome pre - treatment process, and avoid solvent response and sol- vent peak interference during detection. The method was proved to be simple, feasible and effective.