谷氨酰转肽酶合成L-谷氨酰正丙胺

Synthesis of Propyl-L-glutamine by Glutamyltranspeptidase

以L-谷氨酰肼和正丙胺为底物,利用谷氨酰转肽酶合成了L-谷氨酰正丙胺,考察了底物浓度、温度、pH、催化剂用量对L-谷氨酰正丙胺合成的影响。结果表明,谷氨酰转肽酶能有效合成L-谷氨酰正丙胺,在温度37℃、pH=10.0、L-谷氨酰肼浓度为0.4 mol/L、正丙胺浓度为3 mol/L的条件下,谷氨酰转肽酶添加量为0.2 g,300 m L反应体系L-谷氨酰正丙胺的收率为89.7%,经高效液相色谱法测定,L-谷氨酰正丙胺纯度为99.8%。

Glutamyltranspeptidase was used as a catalyst to synthesize propyl-L-glutamine with L-glutamylhydrine and propylamine as substrates. The factors affecting the yield of product such as substrate concentration,temperature,pH,and catalyst dosage were investigated. The results indicated that glutamyltranspeptidase was an efficient catalyst for the synthesis of propyl-L-glutamine,and the optimum reaction conditions were obtained. When the concentration of L-glutamylhydrine was 0.4 mol/L,the concentration of propylamine was 3 mol/L,the catalyst dosage was 0.2 g,the reaction temperature was 37 ℃,and the pH was 10.0,the yield of propyl-L-glutamine was 89.7% in 300 m L reaction volume with a purity of 99.8 % by high performance liquid chromatography.