坡柳种子中坡柳皂苷A的分离鉴定及其对小菜蛾的拒食活性

Isolation and structure identification of dodoneaviscoside A from the seeds of Dodonaea viscosa and its antifeedant activity against Plutella xylostella(L.)

采用小孔树脂、凝胶Sephadex LH-20、反相色谱ODS及制备型高效液相色谱（PreHPLC）等分离技术,从坡柳Dodonaea viscosa（Linn.）Jacq.Enum.种子乙醇提取物中分离获得1个活性化合物。通过质谱及核磁共振等波谱技术,鉴定其为新的齐墩果烷型三萜皂苷类化合物21-epoxyangeloyl-15,16,28-tirhydroxy-22-（2-methylbutanoyl）-Olean-12-en-3-（O-α-Larabinofuranosyl-（1→3）-O-[β-D-glucopyranosyl-（1→2）]-β-D-glucuronide,命名为坡柳皂苷A,英文名dodoneaviscoside A。生物活性测定结果表明,其对小菜蛾Plutella xylostella（L.）3龄幼虫具有较好的非选择性拒食活性,24 h拒食中浓度（AFC50）为207.4μg/m L。值得进一步研究。

A bioactive compound was obtained from the ethanol extract of the Dodonaea viscosa（Linn.） Jacq. Enum. seeds using separation technologies including pore resin, gel Sephadex LH-20,reversed phase chromatography ODS and preparative high performance liquid chromatography（PreHPLC）. The structures of compound 1 were characterized by NMR experiments and mass spectrometry as a new oleanane-type triterpenoid, 21-epoxyangeloyl-15,16,28-tirhydroxy-22-（2-meth-ylbutanoyl）-Olean-12-en-3-（O-α-L-arabinofuranosyl-（1→3）-O-[β-D-glucopyranosyl-（1→2）]-β-D-glucuronide. It was named as dodoneaviscoside A. The results of the bioactivity determination showed that dodoneaviscoside A had antifeedant activity against the 3 th instar larvae of Plutella xylostella（L.） with AFC50 value of 207.4 μg/m L. As a new compound with good nonselective antifeeding activity to P.xylostella, the potential applicatio of dodoneaviscoside A is worth of further investigation.