胎膜早破孕妇胎膜组织中TRAIL、NF-kB的表达及意义

Expression and significance of TRAIL and NF-kB of embryolemma tissue of pregnant women with premature rupture of membrane

目的探究胎膜早破孕妇胎膜组织中肿瘤坏死因子相关凋亡诱导体(TNF-related apoptosisinducing ligand,TRAIL)、核因子-k B(nuclear factor-k B,NF-k B)的表达情况及意义,为胎膜早破的临床诊治提供理论依据。方法选择2015年9月至2016年10月金坛区人民医院妇产科收治的50例胎膜早破孕妇(观察组)与50例正常妊娠晚期孕妇(对照组)为研究对象。采用SP免疫组化法(streptavidin-perosidase,SP)对两组孕妇胎膜组织中TRAIL、NF-k B的蛋白表达进行检测,同时运用聚合酶链式反应检测TRAIL、NF-k B的信使RNA(messenger RNA,m RNA)的表达情况,并进行组间比较。结果观察组孕妇胎膜组织中TRAIL、NF-k B表达的阳性率分别为90.00%和92.00%,对照组为54.00%与62.00%,差异有统计学意义(P<0.05);观察组TRAIL m RNA表达水平为(1.78±0.23),NF-k B m RNA表达水平为(3.68±0.31),均显著高于对照组(1.02±0.14)和(1.88±0.26),差异有统计学意义(P<0.05);TRAIL、NF-k B在胎膜早破孕妇的表达呈正相关(r=0.764,P<0.05)。结论 TRAIL、NF-k B在胎膜早破孕妇中的表达量显著高于正常孕妇,两者参与了胎膜早破的发病过程。TRAIL、NF-k B的正相关关系提示两者可能以某种机制对胎膜早破的发生起促进与调节作用。

Objective To study the expression and significance of TNF related apoptosis inducing ligand （TRAIL） and nuclear factor- kB （NF- kB） of embryolemma tissue of pregnant women with premature rupture of membrane （PROM）, so as to provide theory gist for clinical diagnosis and treatment of PROM. Methods 50 cases of pregnant women with PROM and 50 cases of healthy pregnant women in late pregnancy admitted in Jintan District People＇s Hospital from September 2015 to October 2016 were selected as the object. Pregnant women with PROM were set as observation group, while healthy pregnant women in late pregnancy were set as control group. Stre ptavidin - perosidase（SP） was adopted to all pregnant women for determining protein expression of TRAIL and NF - kB in embryolemma tissue, meanwhile, polymerase chain reaction （PCR） was adopted to determine expression of messenger RNA （mRNA） in TRAIL and NF - kB. Comparison was conducted between the two groups. Results The positive rate of TRAIL and NF - kB expressions in embryolemma tissue of pregnant women in observation group were 90. 00 % and 92. 00 % respectively, while those in control group were 54. 00 % and 62.00 % respectively. Observation group was dramatically higher than control group, with statistical significance（ P 〈 0. 05 ）. The expression level of TRAIL mRNA in observation group was （ 1.78 ± 0. 23）, while that of NF - kB mRNA was （ 3.68 ± O. 31 ）, which were higher than those of control group （ 1.02 ± 0. 14, 1.88± 0. 26 ）, with statistical significance （ P 〈 0.05 ）. There was a positive correlation in expression of TRAIL and NF - kB of pregnant women with PROM （ r = 0. 764,P 〈 0. 05 ）. Conclusion The expression volume of TRAIL and NF - kB of pregnant women with PROM is dramatically higher than that of healthy pregnant women, which proves that these two items are involved in the pathogenesis of PROM. The positive correlation between TRAIL and NF - kB indicates that these two can facilitate or adjust PROM via some mechanism.