PI3K/AKT/mTOR在姜黄素抑制食管癌细胞增殖中的作用

The role of PI3K/AKT/mTOR in the inhibition of curcumin on proliferation of esophageal cancer cells

目的探讨PI3K/AKT/mTOR在姜黄素(Curcumin,Cur)抑制食管癌细胞(Ec-9706)增殖中的作用。方法食管癌Ec-9706细胞给予0、5、10和20μmol/L姜黄素培养24 h、48 h和72 h后,细胞计数CCK-8法检测食管癌Ec-9706细胞的存活率;流式细胞仪法检测Ec-9706细胞凋亡发生;Real-time法和Western blot法检测食管癌Ec-9706细胞中磷酸化蛋白p-PI3K、p-AKT、mTOR、NF-κB和cleaved-Capase-3 m RNA和蛋白表达水平。结果姜黄素可以显著抑制食管癌Ec-9706细胞的增殖和生长,显著降低Ec-9706细胞的存活率,促进食管癌Ec-9706细胞的凋亡,显著升高Ec-9706细胞的凋亡率,且呈时间剂量反应关系,差异有统计学意义(P<0.05)。姜黄素可以显著降低食管癌Ec-9706细胞PI3K、AKT、mTOR m RNA表达和蛋白含量,显著升高NF-κB和cleaved-Capase-3m RNA表达和蛋白含量,且呈时间剂量反应关系,差异有统计学意义(P<0.05)。结论姜黄素可有有效抑制食管癌细胞(Ec-9706)增殖,PI3K/AKT/mTOR信号通路在此过程中起到重要的调节作用。

Objective To investigate the role of PI3 K/AKT/mTOR in the inhibition of the proliferation of esophageal cancer cells（Ec-9706）by curcumin（Cur）. Methods After the cells were treated with 0,5,10 and 20 μmol/L curcumin for24 h,48 h and 72 h,the cell viability was detected by CCK-8 assay in Ec-9706 cells. Flow cytometry was used to detect the apoptosis of Ec-9706 cells. The expression of phosphorylated protein p-PI3 K,p-AKT,mTOR,NF-κB and cleaved-Capase-3 m RNA and protein in esophageal cancer Ec-9706 cells were detected by Real-time and Western blot. Results Curcumin significantly inhibited the proliferation and growth of EC-9706 cells and significantly decreased the survival rate of Ec-9706 cells.Promote the apoptosis of esophageal cancer Ec-9706 cells,and significantly increase the apoptosis rate of Ec-9706 cells in a dose-response manner. The difference was statistically significant（P〈0.05）. Curcumin significantly decreased the expression of PI3 K,AKT and mTOR m RNA and the protein content of esophageal cancer Ec-9706 cells,and significantly increased the expression of NF-κB and cleaved-Capase-3 m RNA and protein,and showed a dose-response relationship with statistical significance（P〈0.05）. Conclusion Curcumin can effectively inhibit the proliferation of esophageal cancer cells（Ec-9706）.The PI3 K/AKT/mTOR signaling pathway plays an important regulatory role in this process.