五裂茶藨子叶的化学成分及其抗炎活性研究

Study on the chemical constituents and the anti-inflammation effects of the leaves of Ribes meyeri

目的研究五裂茶藨子叶中的化学成分,并评价其抗炎活性。方法对五裂茶藨子95%乙醇提取物的乙酸乙酯部位采用硅胶柱层析、MCI柱层析和反相C18制备液相色谱等方法进行分离纯化,根据理化性质和波谱数据鉴定各化合物的结构;采用ELISA法评价所得化合物的抗炎活性。结果从五裂茶藨子叶中分离得到了8个化合物,分别鉴定为鼠李秦素（Ⅰ）、槲皮素-3-O-β-D-葡萄糖苷（Ⅱ）、苜蓿素（Ⅲ）、salcolin A（Ⅳ）、苜蓿素-7-O-β-D-葡萄糖苷（Ⅴ）、阿福豆苷（Ⅵ）、丁香酯素（Ⅶ）、邻苯二甲酸二异丁酯（Ⅷ）;6个黄酮类化合物Ⅰ-Ⅵ均对炎症因子有不同程度的抑制作用,其中,化合物Ⅳ对两种炎症因子的抑制作用最强。结论化合物Ⅰ-Ⅷ均为首次从该植物中分离得到。

OBJECTIVE To study the chemical constituents from the leaves of Ribes meyeri Maxim. and its anti-inflammation bioactivity. METHODS The ethyl acetate fraction of 95% ethanol extracts of Ribes meyeri was isolated and purified by silica gel,MCI and reversed-phase prep C18 chromatographic columns. The structures of obtained compounds were identified by1 HNMR and13 CNMR data. The anti-inflammatory activities of compounds were evaluated by ELISA method. RESULTS Eight compounds were isolated and identified as rhamnazin（ Ⅰ）,quercetin-3-O-β-D-glucoside（ Ⅱ）,tricin（ Ⅲ）,salcolin A（ Ⅳ）,tricin-7-O-β-D-glucopyranoside（ Ⅴ）,kaempferol-3-O-α-L-rhamnopyranoside（ Ⅵ）,（-）-syringaresinol（ Ⅶ） and dibutyl-phthalate（ Ⅷ）.The anti-inflammation bioactivities of compounds Ⅰ-Ⅵ were evaluated by ELISA method. The results showed that all the six compounds have anti-inflammation bioactivity,and compound Ⅳ presented the best bioactivity. CONCLUSION All the compounds have been isolated from Ribes meyeri for the first time.