河南鲁山五大温泉水细菌多样性分析

Bacterial diversity analysis of Five Hot Spring in Henan Lushan

【背景】位于河南中部的平顶山市含有丰富的温泉资源,对温泉水中的微生物多样性和群落结构进行研究有助于更好地开发利用温泉资源。【目的】探究河南鲁山五大温泉水中(上汤、中汤、下汤、温汤和神汤)细菌多样性。【方法】采用Illumina Hi Seq2500 PE250技术对鲁山五大温泉水细菌的16S r RNA基因V3-V4变异区序列进行测序,应用UPARSE、Mothur、Silva、MUSCLE和QIIME等软件整理和统计样品序列数目和操作分类单元(Operational taxonomic unit,OTU)数量,最后分析五大温泉水中细菌的丰度和多样性。【结果】上汤、中汤、下汤、温汤和神汤分别获得56 017、68 319、65 247、59 340、68 825条有效Tags,聚类为670、287、337、598、381个OTU。细菌分类分析表明,五大温泉水的细菌超过40个门,变形菌门(Proteobacteria)占84.12%,是优势菌;在属分类阶元上,五大温泉水的细菌超过239个属,上汤的优势菌是不动杆菌属(Acinetobacter),中汤的优势菌是Tepidimonas,下汤的优势菌是Vogesella和不动杆菌属(Acinetobacter),温汤的优势菌是Sphingopyxis和新鞘脂菌属(Novosphingobium),神汤的优势菌是Aquabacterium。【结论】鲁山五大温泉水中含有丰富的微生物资源,为后续五大温泉水中的微生物资源开发奠定了基础。

[Background] Pingdingshan is rich in hot spring resources. The study of microbial diversity and community structure in the spring water will help us to better exploit these resources. [Objectives] The bacterial diversity of Five Hot Spring（SHANGT, ZHONGT, XIAT, WENT and SHENT） in Henan Lushan was investigated and analyzed. [Methods] The bacterial 16 S r RNA V3-V4 genes were sequenced by Illumina Hi Seq2500 PE250 technology. The OTUs was sorted and counted using UPARSE, Mothur, Silva, MUSCLE and QIIME software. Finally, the bacterial abundance and diversity in Five Hot Spring was analyzed. [Results] It was obtained 56 017, 68 319, 65 247, 59 340, 68 825 effective tags, and acquired 670, 287, 337, 598, 381 OTUs from the SHANGT, ZHONGT, XIAT WENT, and SHENT respectively. The analysis of bacterial classification showed that the bacteria from Five Hot Springs could be classified as more than 40 phyla. Proteobacteria accounted for 84.12% was the dominant bacteria. In the genera level, bacteria from Five Hot Springs could be classified into more than 239 genera. The dominant bacteria of Five Hot Springs（SHANGT, ZHONGT, XIAT, WENT and SHENT） respectively is Acinetobacter, Tepidimonas, Vogesella and Acinetobacter, Sphingopyxis andNovosphingobium, Aquabacterium. [Conclusion] Five Hot Springs in Lushan are rich in microbial resources and may have the potential for the development of such resources.