黄芩配方颗粒与标准汤剂体外抗炎活性的比较

Comparison of the anti-inflammatory activity of Huangqin dispensing granules and Huangqin standard decoction

目的:研究黄芩配方颗粒与黄芩标准汤剂的体外抗炎活性,评价两者的药效等效性。方法:在HPLC法对黄芩配方颗粒与黄芩标准汤剂中黄芩苷、汉黄芩苷和黄芩素的含量进行表征的基础上,采用脂多糖诱导小鼠巨噬细胞RAW264.7细胞建立炎症模型,以WST-1法和Griess法分别考察了10批次黄芩配方颗粒及黄芩标准汤剂的细胞毒性及对NO生成量的影响,然后采用酶联免疫吸附法检测上述细胞上清液中炎症因子TNF-α和IL-1β的浓度,评价黄芩配方颗粒和标准汤剂的抗炎活性。结果:10批次黄芩配方颗粒与黄芩标准汤剂中黄芩苷、汉黄芩苷和黄芩素的含量均无显著性差异,且对RAW264.7细胞无明显细胞毒性(50 mg·L-1)。与正常对照组相比,细菌脂多糖(LPS)显著诱导了巨噬细胞中NO,TNF-α及IL-1β(P<0.01)的分泌;与LPS组比较,50 mg·L-1的黄芩配方颗粒显著降低了细胞NO,TNF-α和IL-1β的水平(P<0.01),且与相同剂量的黄芩标准汤剂相比,抗炎效果没有显著性差异。结论:黄芩配方颗粒与黄芩标准汤剂的主要化学成分具有一致性,均可显著抑制LPS诱导的RAW264.7细胞释放NO,TNF-α和IL-1β,具有体外抗炎等效性,可为中药配方颗粒的研究和应用提供参考依据。

Objective： This study aimed to evaluate the efficacy equivalence of Huangqin dispensing granule and Huangqin standard decoction by investigating their anti-inflammatory activities in vitro. Methods：Based on the characterization of baicalin,wogonoside,baicalein content in Huangqin dispensing granule and Huangqin standard decoction by HPLC,the inflammatory model was established by stimulating mouse macrophage RAW264. 7 cells with LPS. The cytotoxicity and the effect on cell NO production of 10 batches of Huangqin dispensing granule and Huangqin standard decoction were determined by WST-1 and Griess method respectively.Then,the concentration expression of TNF-α and IL-1β in cell supernatant was detected by ELISA to evaluate theiranti-inflammatory activities. Results： There was no significant difference in the content of baicalin,wogonoside,baicalein in 10 batches of Huangqin dispensing granule and Huangqin standard decoction,which also exhibited no obvious cytotoxicity to RAW264. 7 cells at 50 mg·L-1. Compared with the normal control group,LPS significantly induced the secretion of NO,TNF-α and IL-1β（ P 0. 01） in macrophages. Compared with LPS group,Huangqin dispensing granule at 50 mg·L-1 significantly decreased the levels of NO,TNF-α and IL-1β（ P 0. 01）,there is no significant difference compared with Huangqin standard decoction. Conclusion： There was no significant difference between the main chemical content of Huangqin dispensing granule and that of Huangqin standard decoction,which both significantly inhibited the release of NO,TNF-α and IL-1β in LPS-stimulated RAW264. 7 cells. It indicated that Huangqin dispensing granule and Huangqin standard decoction had the equivalent anti-inflammatory efficacy in vitro,which provided a reference for the application of TCM dispensing granule.