气相色谱仪-质谱指纹图谱应用与烟用香精香料品质控制

Quality Control in Tobacco Flavor by Gc/Ms Combined with Infrared Spectrum

烟用香精香料化学成分复杂,本文采用3种不同前处理方式:固相微萃取,同时蒸馏萃取和溶剂萃取法进行提取,在比较了他们之间的效果后,采用溶剂萃取法和气相色谱仪-质谱相结合的方法建立一种新的色谱指纹图谱方法,通过相似度比较和成分分析,该指纹图谱完全能够应用于香精的质量控制。

A new chromatographic fingerprinting method has been established for quality control of tobacco flavor. Three different extraction techniques, solid-phase, simultaneous distillation extraction and solvent extraction were evaluated for isolation of the component of interest. After comparison of their performance, a combination of solvent extraction and GC-MS was used for simple, reliable, and reproducible development of a chromatographic fingerprint of tobacco flavor. The chromatographic fingerprint developed was successfully used to differentiate tobacco flavor samples from tobacco extract prepared from tobacco leaf, both by similarity comparison and by principal-components projection analysis. The method can be used for quality control of tobacco flavor.