无芒雀麦组织培养再生体系的建立

Tissue culture and regeneration of Bromus inermis

以无芒雀麦(Bromus inermis)的成熟种子为外植体,建立其组织培养再生体系,筛选最适愈伤组织诱导、分化及再生植株生根培养基,为之后的转基因实验奠定基础。结果表明:MS+1 mg·L^(-1) 2,4-二氯苯氧乙酸(2,4-D)+0.5 mg·L^(-1) 6-苄氨基腺嘌呤(6-BA)为最佳愈伤组织诱导培养基,诱导率为68.7%;MS+2 mg·L^(-1)萘乙酸(NAA)+1 mg·L^(-1) 6-BA为最佳分化培养基,分化率高达100%;不添加任何植物生长调节物质的1/2MS培养基为最佳生根培养基;组培苗移栽至蛭石与珍珠岩3:1(V/V)的基质中,30 d后成活率可达70%~80%。

Tissue culture and regeneration of Bromus inermis were established using the seeds as explants.The optimal induction,differentiation and rooting media were selected for subsequent transgenic experiment.The results show that the optimal medium for callus induction was MS＋1 mg·L^-1 2,4-dichlorophenoxyacetic acid（2,4-D）＋0.5 mg·L^-1 6-benzylaminopurine（6-BA）,and callus induction ratio reached to 68.7%.The optimal medium for adventitious bud differentiation was MS＋2 mg·L^-1 1-naphthylacetic acid（NAA）＋1 mg·L^-1 6-BA,and the differentiation ratio was 100.0%.The optimal rooting medium was 1/2MS.Regenerated plantlets were transplanted into the culture matrix with vermiculite and perlite（3：1,V/V）,and the survival rate was 70%-80%after 30 d.