加拿大一枝黄花茎秆木质素含量和木质素化程度的测定方法

Methods for determination of stem lignin content and lignification of Solidago canadensis

测定木质素含量的方法主要有Klason法和巯基乙酸(TGA)法,其中Klason法测定类型较全面,而TGA法主要用于测定β-O-4型木质素含量。Fasga染色法和Quantifasga法可用于观察和测定木质部和韧皮部木质素化程度。为了探究上述方法是否能用于测定加拿大一枝黄花(Solidago canadensis)茎秆木质素含量和木质素化程度,为进一步研究木质素与其入侵能力的关系提供试验方法,本研究选用采集于南京、南通和宁波3个地区的加拿大一枝黄花种群,在生长期和成熟期分别测定了它们的株高和茎直径;利用上述方法测定其茎秆木质素含量及木质部和韧皮部木质素化程度。结果显示,南京种群成熟期株高、茎直径均显著高于其他2个种群。Klason法、TGA法的测定结果显示,3个地理种群生长期的木质素含量没有显著差异;且随着生长发育期的延长均显著增加;成熟期南通种群具有最高的总木质素含量。Fasga染色法和Quantifasga法测定结果显示,在生长期各地理种群木质部和韧皮部木质素化发育程度相似;但在成熟期,南京种群木质部木质素化程度显著高于宁波种群。上述结果表明,Klason法和TGA法都能有效测定加拿大一枝黄花木质素含量,由于测定的木质素类型不同,建议根据实验目的选择;Fasga染色法及Quantifasga法能应用于加拿大一枝黄花茎秆木质素化程度的定性和定量研究。

Klason method and thioglycolic acid method（TGA） have been widely used to measure lignin content in plants, in which the former for total lignin content, the latter for the lignin content of β-O-4 mainly. Fasga staining method and Quantifasga method are used to determine lignification in stem. In order to explore whether these methods can be used to determine the stem lignin content and lignification of Solidago canadensis, and provide the methods for studying the relationship between stem lignin contents as well as lignification and invasive ability of S. canadensis, three populations collected from Nanjing, Nantong and Ningbo were selected as experimental materials. The plant height and stem diameter were measured in growing and mature stages, respectively. The lignin content of stem was determined and lignification in xylem and phloem was analyzed by the above methods. The results showed that the height and stem diameter of population from Nanjing were significantly higher than other two populations in mature stage. The lignin contents had no significant differences among 3 populations in growing stage by Klason and TGA methods, and the lignin contents increased significantly in three populations with growth. In mature stage, population from Nantong had the highest total lignin content. Three populations showed similar stem lignin distribution in growing stage, while population from Nanjing showed a significantly higher lignification of xylem than population from Ningbo in mature stage. These results suggested that both methods of Klason and TGA can be used effectively to determine the lignin content in the stem of S. canadensis, and should be chosen based on the experimental aim. Fasga staining method and Quantifasga method can be applied to study on lignification in the stem of S. canadensis qualitatively and quantitatively.