远志寡糖酯抗β淀粉样蛋白25-35诱导的SH-SY5Y细胞损伤的作用研究

Effects of Oligosaccharide Esters of Polygala tenuifolia on the Injury of Human Neuroblastoma SH-SY5Y Cells Induced by Aβ_(25-35) Fragment

目的探讨远志寡糖酯抗β淀粉样蛋白25-35诱导SH-SY5Y细胞损伤的作用及相关机制。方法不同浓度的远志寡糖酯与SH-SY5Y细胞孵育1 h,再与25μmol·L^(-1)的βAmyloid Fragment_(25-35)(Aβ_(25-35))共同孵育24 h。四甲基偶氮唑蓝(MTT)法检测细胞存活率;流式细胞仪检测细胞凋亡;生化法测定细胞上清液中乳酸脱氢酶(LDH)含量及细胞内超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量;Western blot检测胞内凋亡相关蛋白Bcl-2和Bax的表达。结果远志寡糖酯能够显著提高Aβ_(25-35)损伤的SH-SY5Y细胞存活率,降低细胞凋亡率,减少细胞LDH释放,增加胞内SOD活性并降低MDA含量,增加抗凋亡蛋白Bcl-2的表达,降低凋亡蛋白Bax的表达。结论远志寡糖酯能够对抗Aβ_(25-35)诱导的SH-SY5Y细胞损伤,与抗氧化损伤和减少细胞凋亡有关。

OBJECTIVE To investigate the effects of oligosaccharide esters of Polygala tenuifolia on Aβ25-35 induced injury in SH- SY5Y cells and the relative mechanisms. METHODS SH-SY5Y cells were cultured with Aβ25-35 （25 μmol · L^-1 ） for 24 h in the presence and absence of oligosaccharide esters component of Polygala tenuifolia. The cell viability was measured by MTT method ; cell apoptosis was detected by flow cytometry; the LDH release, the SOD activity and MDA level were measured by biochemistry method; the Bcl-2 and Bax expression were determined by Western blotting. RESULTS Oligosaccharide esters of Polygala tenuifolia could enhance the cell viability, decrease the apoptotic rate, the release of LDH and MDA level, and increase SOD activity. It also enhanced the expression of anti-apoptotic protein Bcl-2 and decreased the expression of apoptotic protein Bax. CONCLUSION Oligosaccharide esters of Polygala tenuifolia have neuroprotective effects against the Aβ25-35 induced injury in SH-SY5Y cells, which is related to its anti-oxidation and anti-apoptosis.