金银花叶中绿原酸的提取及抗氧化活性研究

Study on the Extraction and Antioxidant Activity of Chlorogenic Acid from Lonicera japonica Leaf

本研究利用双水相体系分离金银花叶中的绿原酸并分析其抗氧化活性。探讨4种乙醇/无机盐双水相体系提取绿原酸的能力,在乙醇浓度、盐浓度、提取时间和温度4个单因素实验的基础上,通过响应面法研究Na H_2PO_4添加量、乙醇浓度和提取时间3因素及其交互作用对绿原酸提取率的影响。研究结果显示,4种乙醇/无机盐双水相体系中,乙醇/Na H_2PO_4双水相体系具有最佳的绿原酸提取效果。优化的提取工艺为Na H_2PO_4添加量3.15 g、乙醇浓度28.5%和提取时间49 min,提取率可达80.2%。当金银花叶绿原酸浓度为0.5 mg/m L时,其DPPH自由基清除率达84.2%;绿原酸浓度为1 mg/m L时,脂肪过氧化抑制能力达88%。这些研究结果表明乙醇/Na H_2PO_4双水相体系能有效提取金银花叶中的绿原酸,具有较好的应用前景。

In this paper, an aqueous two - phase system （ ATPS ） was used in separation of chlorogenic acid from Lonicera japonica leaf and the its antioxidant activity of the chlonogeni acid was studied. Four different ethanol/inorganic salt systems were adapted to extract the chlorogenic acid, and the effects of ethanol concentration, salt content, extract time and extract temperature on the extraction efficiency of chlorogenic acid were discussed in detail.Also, response surface design was employed to evaluate the effect of ethanol concentration, extraction time, NaH2PO4 addition and their interactions.The results showed that the aqueous two-phase system（ ethanol/NaH2PO4 ）had the best extraction effect. The optimal conditions for chlorogenic acid extraction were that ,3.15 g Nail2 PO4,28.5 % ethanol concentration and 49 min extraction time.The chlorogenic acid yield under the optimal conditions was 80.2% .The scavenging ability of the chlorogenic acid reached 84.2% at 0.5 mg/mL against DPPH radical, and the inhibitory effect of lipid peroxidation attained 88% at 1 mg/mL.These results suggested that the aqueous twophase system（ ethanol/NaH2PO4）was efficient in extracting chlorogenic acid from Lonicera japonica leaf and had the potential to be used in chlorogenic acid extraction industry.