摘要
目的:鉴定一种经分离纯化得到的姬松茸多糖的纯度,并分析其单糖组成。方法:采用苯酚-硫酸法测定姬松茸多糖总糖含量,结合凝胶过滤法、高效液相色谱-蒸发光散射检测(HPLC-ELSD)法和紫外可见吸收光谱法鉴定其纯度,运用高效液相色谱-柱前衍生化法对其单糖组成进行定性、定量分析。结果:所得姬松茸多糖样品的多糖含量为99.50%±2.5%(W/W,以葡萄糖计),且不含核酸、蛋白质等杂质,纯度较高,其单糖组成为葡萄糖、半乳糖、甘露糖(含量比分别为99.2%、0.6%、0.2%)。结论:所得姬松茸多糖是一种纯度较高、葡萄糖为主要单糖的均一多糖。
Objective: To identify the purity and analyze the monosaccharide composition of a polysaccharide isolated and purified from Agaricus brasiliensis.Methods:The polysaceharide content of Agaricus brasiliensis polysaccharide was determined by phenol-sulfuric acid method, and its purity was identified by Sephadex Filtra, High-Performance Liquid Chromatography- Evaporative Light Scattering Detection ( HPLC - ELSD ) method and UV - Visible Absorption Spectroscopy. High Performance Liquid Chromatography (HPLC)- Column Derivatization method was used to determine the monosaeeharide composition of the polysaccharide.Results :The purity of the obtained polysaccharide was high : its polysaecharide content was 99.50% - 2.5% ( W/W, calculated as glucose), and it does not contain nucleic acid, protein and other substances. The polysaccharide was mainly composed of mannose, glucose and galactose, which accounts for 0.2% , 99.2% and 0.6%, respectively. Conclusion: The obtained Agaricus brasiliensis polysaccharide was a homogeneous polysaccharide with high purity and more glucose content.
作者
房雷雷
赵肖通
辛慧洁
林婷婷
张彦青
解军波
FANG Lei-lei;ZHAO Xiao-tong;XIN Hui-jie;LIN Ting- ting;ZHANG Yan-qing;XIE Jun-bo(School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134, China;Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第13期94-97,267,共5页
Science and Technology of Food Industry
基金
2015年天津市"131"创新型人才培养工程第一层次人才计划项目
关键词
姬松茸多糖
纯度
单糖组成
PMP
Agaricus brasiliensis polysaccharidc
purity
monosaccharide composition
PMP