桑皮素对人前列腺癌Du145细胞的抑制作用

Inhibiting effect of mulberin on human prostate cancer cell Du145

目的:为探讨桑皮素对Du145细胞的增殖抑制及其诱导凋亡的影响。方法:用5、10和15μmol/L的桑皮素作用于Du145细胞24 h后,在倒置显微镜及透射电镜下观察细胞形态学变化;利用CCK-8法来检测桑皮素对Du145细胞的增殖的影响;应用流式细胞术和DNA fragmentation实验检测Du145细胞的凋亡情况;采用Western blot技术检测Gli1和VEGF蛋白的表达变化。结果:发现桑皮素能够抑制Du145细胞的生长,并诱导Du145细胞发生凋亡甚至死亡,作用24h后IC50=16.5μmol/L;与对照组相比,细胞凋亡率显著升高,当作用浓度≥15μmol/L后细胞晚期凋亡明显增多;随着桑皮素作用浓度的增加,Gli1和VEGF蛋白表达量明显下降。结论:桑皮素诱导Du145细胞的凋亡,并降低了Hh信号通路中Gli1和VEGF因子的表达。

Objective： To investigate the proliferation inhibition and inducing apoptosis of mulberin on Du145 cells. Methods： Du145 cells were treated with 5,10,15μmol/L mulberin for 24 hours,morphological changes of cells were observed under inverted microscope and transmission electron microscopy. The inhibitory effect of mulberin on Du145 cells was detected by CCK-8 method. The apoptosis of Du145 cells was detected by flow cytometry and DNA fragmentation. Expressions of Gli1 and VEGF protein were detected with Western blot. Results： Mulberin inhibited the growth of Du145 cells,and it induced the apoptosis even death of Du145 cells（ when IC50= 16. 5 μmol/L after 24 hours）. Compared with the control group,the apoptosis rate of Du145 cells was significantly increased. And the late apoptosis obvious increased when the concentration was ≥ 15 μmol/L. With the increase of the concentration of mulberin,protein expressions of Gli1 and VEGF significantly decreased. Conclusion： Mulberin can induce the apoptosis of Du145 cells,and it can decrease expressions of Gli1 and VEGF factors in the Hh signaling pathway.