甘蔗野生种质资源抗褐锈病基因Bru1的分子检测

Molecular Detection of Brown Brust Resistance Gene Bru1 in Wild Germplasm Resources of Sugarcane

在甘蔗栽培品种‘R570’上发现和定位的甘蔗抗褐锈病主效基因Bru1对世界上不同国家和不同地区的黑顶柄锈菌具有广谱抗性。针对该基因开发的两个分子标记能有效、稳定地检测和筛选含有该基因的种质资源。为筛选甘蔗野生种抗源种质,利用R12H16和9O20-F4两个标记对云南农业大学甘蔗种质资源圃保存的部分野生种质资源进行抗褐锈病基因Bru1的分子检测,包括割手密96份、蔗茅15份、滇蔗茅1份、斑茅11份、河八王4份、金茅2份、五节芒9份,共计138份。结果表明,有7份割手密含抗褐锈病基因Bru1,占参试材料的5.1%。本研究为甘蔗的转基因育种和抗褐锈病杂交育种提供优良抗源材料,为进一步开展甘蔗育种研究提供了科学依据。

The major gene Bru1, which is found in the sugarcane cultivar‘R570＇, is resistant to brown rust, and it has broad spectrum resistance to brown rust disease isolated in sugarcane of different countries and regions. The two molecular markers developed according to this gene can efficiently and stably detect and screen germplasm resources that containing the gene. For the screening of wild resistant germplasms of sugarcane, 138 wild sugarcane clones（including 96 clones of S. spontaneum, 15 clones of E. fulvus, 1 clone of E. rockii,11 clones of E. arundinaceus, 4 clones of‘N.porphyrocoma＇, 2 clones of‘E.speciosa＇, 9 clones of M. floridulus） that preserved in sugarcane germplasm resources of Yunnan Agricultural University were detected using markers R12 H16 and 9 O20-F4. The results showed that Bru1 was detected in seven Saccharum spontaneum clones, which accounted for 5.1% of the tested materials. This study provided excellent resistance materials for transgenic breeding of sugarcane and cross breeding of brown rust, and laid the foundation for further research on sugarcane breeding.