基于SSR标记的葡萄品种鉴别和指纹图谱构建

Identification and Fingerprinting Construction of Vitis vinifera Variety Based on SSR Markers

本研究基于SSR标记技术,对山西省农业科学院果树研究所自育葡萄品种及其亲本进行分子鉴别,并建立其对应的指纹图谱。供试材料为山西省农业科学院果树研究所9个自育葡萄品种及其7个亲本,采用国际通用的SSR荧光标记引物进行扩增,毛细管电泳进行基因分型,品种鉴别及构建指纹图谱。从44对SSR引物中筛选出15对特异性引物用于随后的SSR扩增,共扩增出56条条带,其中多态性条带51条,多态性百分率为94.4%。SSR扩增结果分析表明,16份葡萄种质遗传距离的变异范围为0.068 7~0.919 1。UPGMA聚类分析表明,15对SSR标记不仅可用于不同亲本、不同种群、不同亚属间的亲缘关系鉴定,更可用于相同亲本近缘姊妹系品种及其亲本间的鉴别。该SSR指纹图谱的建立,可为中国葡萄品种的种质资源数据库的完善,近缘品种鉴别以及品种保护提供参考依据。

This study carried out the molecular identification of Vitis vinifera varieties and their parents cultivated by Pomology Institute, Shanxi Academy of Agricultural Sciences and constructed their corresponding fingerprint based on SSR marker technology. As test materials, 9 grape varieties cultivated by Pomology Institute, Shanxi Academy of Agricultural Sciences and their 7 parents were amplified by international SSR fluorescent marker primer, and the method of capillary electrophoresis was used for genotyping, variety identification and fingerprint construction. 15 pairs of specific primers were screened from 44 pairs of SSR primers, which were used for the following SSR amplification. A total of 56 bands were amplified, including 51 polymorphic bands with 94.4%polymorphic percentage. According to the analysis of SSR amplification result, the variation range of genetic distance of 16 grape germplasms was between 0.068 7 and 0.919 1. The UPGMA clustering analysis showed that the 15 SSR markers could be applied to the identify the relationship between different parents, different populations, and different subspecies. At the same time, it also could be applied for the identification of the homologous proximal sister species and their parents. The SSR fingerprinting construction would provide a good reference for the improvement of germplasm resources database of Chinese Vitis vinifera varieties, the identification of closely related varieties and new varieties protection.