日本结缕草(Zoysia japonica)修剪后组织与器官再生过程的分子机制

Molecular Mechanism of Tissue and Organs Regeneration after Cutting in Zoysia japonica

为了探究日本结缕草(Zoysia japonica)修剪后组织与器官再生过程的分子机制,分析器官再生相关植物激素生长素(IAA)和细胞分裂素(CTK)对这一过程的影响。本研究对修剪后0、2 h、6 h、24 h的日本结缕草品种‘Zenith’进行拍照和显微观察,以分析其形态与茎尖分生组织显微结构变化。利用酶联免疫吸附测定法(ELISA)和实时荧光定量PCR(qRT-PCR)技术来探究IAA、CTK及其相关基因的响应模式。结果显示ZjPIN(PIN-FORMED1)调控的IAA极性运输在修剪后发生响应,参与日本结缕草茎尖分生组织2 h和24 h器官原基的形成,而IAA信号转导途径在6 h后激活,参与植株的快速生长。ZjWUS(WUSCHEL)在2 h和24 h参与茎尖分生组织中细胞的增殖,而CTK信号在6 h时开始活跃,抑制ZjWUS的表达,维持茎尖分生组织的稳定。这些结果说明ZjPIN1和ZjWUS协同IAA和CTK的信号转导途径参与了日本结缕草修剪后的组织和器官再生过程。该研究为植物损伤后组织与器官再生过程分子机制的研究提供了理论依据。

In order to explore the molecular mech anism of Zoysia japonica tissue and organ regeneration after cutting, and analyze the effects of auxin（IAA） and cytokinin（CTK） on this process, Z. japonica cultivar‘Zenith＇at 0 h, 2 h, 6 h and 24 h after cutting was photoed and observed by microscope to analyze its morphology and microstructural changes in stem apex meristem. Enzyme-linked immunosorbent assay（ELISA） and real-time fluorescent quantitative PCR（qRT-PCR） were used to explore IAA, CTK and the response models of related genes. The results showed that the polar transport of IAA regulated by ZjPIN1 responded after cutting, which was involved in the formation of organ primordia at 2 h and 24 h in the SAM of ‘Zenith＇, and IAA signal transduction pathway was activated after 6 h, and involved in the rapid growth of plants. The ZjWUS gene participated in the proliferation of SAM at 2 h and 24 h, whereas CTK signal became active at 6 h, which inhibited the expression of ZjWUS and maintained the stability of SAM. These results indicated that ZjPIN1 and ZjWUS, combined with IAA and CTK signal transduction pathways, participated in tissue and organ regeneration of Zoysia japonica after cutting. This study could provide a theoretical basis for the study of the molecular mechanism of plant tissue and organ regeneration after injury.