响应面法优化火龙果茎甾醇的提取及纯化工艺研究

Optimization of extraction and purification of phytosterol from pitaya stem by response surface methodology

以火龙果茎为原料,研究火龙果茎甾醇的提取及精制工艺。利用响应面分析法对火龙果茎甾醇超声提取工艺进行优化。结果表明：超声波辅助提取火龙果茎甾醇的最佳工艺条件为提取时间57 min、超声功率143 W、液料比12∶1（m L/g）、温度53℃,甾醇得率为2.693‰。通过正交试验确定火龙果茎甾醇精制的最佳条件为：液料比为4∶1（m L/mg）,结晶初始温度为55℃,养晶时间为18 h,此条件下火龙果茎甾醇的纯度达到87.6%。

In this study,the extraction and purification technologies of phytosterol from pitaya stem were investigated. The response surface methodology was used to optimize ultrasound-assisted extraction of phytosterol from pitaya stem. The result showed that the best ultrasonic extraction conditions were as follows：the ultrasonic time was 57 min,the ultrasonic power was 143 W,the ultrasonic temperature was 53℃,and the liquid to solid ratio was 12∶1（m L/g）.The total phytosterol extraction yield was 2.693‰. The optimum purification conditions of phytosterol from pitaya stem were determined by the orthogonal experiment：the liquid to solid ratio was 4∶1（m L/mg）,initial crystallization temperature was 55 ℃,and crystallization time was 18 h. The purity of phytosterol from pitaya stem reached up to 87.6% under the optimum conditions.