髋关节正常软骨与骨性关节炎软骨相关因子表达的对照研究

Comparative Study of the Expression of Cartilage Related Factors in the Normal Cartilage and Osteoarthritis in the Hip Joint

目的:对人髋关节正常关节软骨和骨关节炎关节软骨进行对比研究,对骨性关节炎生物学特性及炎症因子变化进行比较和评价。方法:选取股骨颈骨折排除关节炎的病例及骨性关节炎病例各22例,分为2组,正常关节软骨组及骨性关节炎软骨组。1)大体及组织染色的方法(甲苯胺蓝和Masson染色)观察骨性关节炎组及正常软骨组的组织形态、糖胺多糖及胶原蛋白含量。2)Western blot检测两组关节软骨中CollagenⅡ,Aggrecan,MMP-13,Caspase3和Cleaved caspase3蛋白表达量。3)ELISA法检测两组关节液中TNF-α的表达水平。结果:1)两组组织染色分别显示蛋白多糖和胶原纤维含量:骨性关节炎组比正常关节软骨组均明显减少。2)Western blot检测显示MMP-13,Caspase3和Cleaved caspase3各蛋白含量在骨性关节炎组中明显升高,差异有统计学意义(P<0.05)。3)Western blot检测显示Ⅱ型胶原和蛋白多糖蛋白表达量在骨性关节炎组中明显下降,差异有统计学意义(P<0.05)。4)ELISA法检测提示骨性关节炎组关节液中TNF-α的表达水平较正常组明显上升,差异有统计学意义(P<0.05)。结论:骨性关节炎的病理过程是多种炎症因子调节下引起关节软骨细胞的凋亡,进而引起的软骨基质成分合成-分解代谢的平衡破坏的过程。

Objective：To compare and evaluate the biological characteristics and inflammatory factors of human normal cartilage and osteoarthritis.Methods：Toluidine blue and Masson staining were observed of glycosaminoglycan and collagen content,from the protein level detection of two groups of Collagen Ⅱ,aggrecan,MMP-13,caspase 3 and cleaved caspase 3 protein level and ELISA method to detect the expression level of TNF-alpha in synovial fluid.Results：1）Masson staining and toluidine blue staining showed that O proteoglycan and collagen fiber content in osteoarthritic cartilage was weaker.2）Western blot showed that the levels of MMP-13,caspase 3 and cleaved caspase 3 protein increased significantly in articular cartilage of osteoarthritis（P〈0.05）.3）Western blot showed that the expression of type Ⅱ collagen and proteoglycan in joint cartilage of osteoarthritis was less than that in normal cartilage（P〈0.05）.4）ELISA showed that the expression level of TNF-alpha in bone joint fluid was increased significantly（P〈0.05）.Conclusion：We believe that the pathological process of osteoarthritis is caused by the apoptosis of articular chondrocytes under the regulation of a variety of inflammatory factors,and then leads to the process of balance and destruction of the synthesis and catabolism of cartilage matrix components.