痛泻要方对急性放射性肠炎大鼠肠组织的防护作用及机制

Protective effect of Tongxie Yaofang formula on intestinal tissue of rats with acute radiation enteritis

目的初步研究痛泻要方对急性放射性肠炎(radiation enteritis,RE)大鼠肠组织的防护作用及机制.方法随机将48只♂SD大鼠分为4组:A组为正常对照组(n=12)、B组为模型照射组(n=12)、C组为痛泻要方组(n=12)、D组为谷氨酰胺组(n=12);A组不予任何处理,其余三组均以高能X线直线加速器给予10 Gy全腹腔照射.造模成功后第1天,A组和B组给予蒸馏水,C组给予中药痛泻要方,D组给予谷氨酰胺,各组均连续灌胃7 d.每日观察大鼠一般状况、排便情况及体重变化.各组均于灌胃结束后6 h取空肠组织,光镜下观察肠组织形态学变化.检测空肠组织匀浆一氧化氮(nitric oxide,NO)的含量,ELISA法测定空肠黏膜炎性因子白介素(interleukin,IL)-6、IL-10、肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)的蛋白含量.结果灌胃结束后,除B组于造模第3天死亡1只外,其余各组均无死亡.C组和D组大鼠的一般状况及黏液血便均有不同程度改善,体重增加较B组明显(P<0.05).与B组比较,C组和D组大鼠的NO、IL-6及TNF-α含量均出现下降(P<0.05).C组和D组大鼠的IL-10含量较B组大鼠明显升高,差异均有显著统计学意义(P<0.01).结论痛泻要方对急性RE大鼠的肠黏膜具有防护作用,其机制可能与降低空肠NO、IL-6及TNF-α活性,提高IL-10含量,减轻肠组织炎症反应有关.

AIM To investigate the protective effect of Tongxie Yaofang formula on intestinal tissue of rats with acute radiation enteritis （RE） and mechanisms. to explore the underlying METHODS Forty-eight Sprague-Dawley （SD） male rats were randomly divided into four groups： A-D. Group A （n = 12） was given no treatment, while group B （n = 12）, group C （n = 12）, and group D （n = 12） underwent whole abdominal irradiation at a single dose of 10 Gy. At day 1 after irradiation, group A and group B were given distilled water, while group C were given Tongxie Yaofang formula and group D were given glutamine by gavage for 17 consecutive days. The general condition, defecation, and weight change of rats were daily observed. In each group, jejunal tissue was taken at 6 h after gastric lavage, and the morphology of intestinal tissue was observed under a light microscope. The content of nitric oxide （NO） in jejunal tissue was measured, and the protein contents of interleukin （IL）-6, IL-10, and tumor necrosis factor α （TNF-α） were determined by ELISA.RESULTS After gastric lavage, one rat died at day 3, and no death was found in all the other groups. Rats in group C and group D had different degrees of improvement in general condition and mucosanguineous feces. The body weight of rats in group C and group D increased significantly compared with that of group B （P 〈 0.05）. Compared with group B, the contents of NO, IL-6, and TNF-α in group C and group D significantly decreased （P 〈 0.05）. The levels of IL-10 in group C and group D were significantly higher than that in group B （P 〈 0.01）.CONCLUSION Tongxie Yaofang formula has a protective effect on intestinal tissue of rats with acute RE possibly via mechanisms that may be related to decreasing the levels of NO, IL-6, and TNF-α in jejunum, increasing the content of IL-10, and reducing the inflammation in intestinal tissue.