摘要
A facile approach has been developed to synthesize Fe3O4@PAM（polyacrylamide） nanoparticles（NPs） with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe3O4 NPs aqueous suspension. Nitrilotriacetic acid（NTA） was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni^（2＋） ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins（His-tagged GFP）. The high magnetic property of Fe3O4@PAM@NTA-Ni^（2＋） allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity（42 μg protein/mg of NPs）. The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale.
A facile approach has been developed to synthesize Fe3O4@PAM（polyacrylamide） nanoparticles（NPs） with carboxyl groups on the surfaces by copolymerization with acrylamide and acrylic acid in Fe3O4 NPs aqueous suspension. Nitrilotriacetic acid（NTA） was conjugated to the magnetic NPs via well-known carboniimide chemistry using EDC and NHS. The Ni^（2＋） ions loaded on the surface of NPs provide abundant docking sites for immobilization of His-tagged green fluorescent proteins（His-tagged GFP）. The high magnetic property of Fe3O4@PAM@NTA-Ni^（2＋） allows an easy separation of the NPs from solution under an external magnetic field, with high His-tagged protein binding capacity（42 μg protein/mg of NPs）. The NPs can be recycled for at least four times without significant loss of binding capacity to proteins. These materials show great potential to separate His-tagged protein with low-cost purification at industrial scale.
基金
Funded by the National Natural Science Foundation of China(Nos.21401051 and 51303049)
Hubei Province Natural Science Foundation of China(Nos.2014CFB595 and 2014CFA080)
Chutian Scholars Fund Project from the Education Department of Hubei Province
Hundred Talents Program from the Organization Department of Hubei Province
