MiR-194-3p对成纤维细胞的增殖作用研究

The effect of miR-194-3p on the proliferation of fibroblasts

目的 研究瘢痕疙瘩中miR-194-3p对成纤维细胞增殖作用的影响,为瘢痕疙瘩的治疗提供理论基础。方法 对瘢痕疙瘩标本应用基因芯片筛选出与正常组织差异表达的miRNA,并通过real-time PCR技术来验证miR-194-3p表达;采用Western-blot和real-time PCR分析miR-194-3p对其靶蛋白RUNX2的调节作用。在成纤维细胞中过表达或抑制miR-194-3p后,通过MTT实验观察其对成纤维细胞增殖的作用。通过Western-blot和real-time PCR探讨其对RUNX2及其增殖相关蛋白CDK4的调控作用。结果 miR-194-3p在瘢痕疙瘩中呈下调表达,其能够抑制RUNX2的表达;miR-194-3p可以通过抑制增殖相关蛋白CDK4来抑制成纤维细胞的增殖。结论 miR-194-3p可以抑制瘢痕疙瘩成纤维细胞的增殖。

Objective To analyze the effects of miR-194-3p on the proliferation of fibroblasts in keloids, and provide a theoretical basis for the treatment of keloids. Methods The differential expression of miRNAs with normal tissue was screened by microarray analysis on keloids. Real-time PCR technique was used to verify the expression of miR-194-3p. Western blot and real-time PCR were used to analyze the regulatory effect of miR-194-3p on its target protein RUNX2. After overexpression or inhibition of miR-194-3p in fibroblasts, the proliferation of fibroblasts was observed by MTT experiment. Western blot and real time PCR were used to investigate the regulation of RUNX2 and CDK4, its proliferation-related protein. Results The expression of miR-194-3p was down-regulated in keloids, and it was able to inhibit the expression of RUNX2. MiR-194-3p was able to inhibit the proliferation of fibroblasts by inhibiting CDK4, the proliferation-related protein. Conclusion MiR-194-3p can inhibit the proliferation of keloid fibroblasts.