响应面法优化椿皮中铁屎米-6-酮生物碱超声辅助提取工艺

Optimization of Ultrasound-assisted Extraction Technology of Canthin-6-one from Bark of Ailanthus Altissima by Response Surface Methodology

目的应用响应面法优化超声辅助提取椿皮中铁屎米-6-酮生物碱的工艺条件。方法以铁屎米-6-酮生物碱提取量为指标,在单因素考察乙醇提取铁屎米-6-酮的基础上,采用中心组合试验设计和响应面分析法优选提取工艺参数。结果最佳提取工艺条件为乙醇浓度68%,液固比32 m L·g^(-1),超声温度54℃,超声时间38 min,铁屎米-6-酮生物碱提取量为26.19μg·g^(-1),接近于模型预测值(26.31μg·g^(-1))。结论优选的超声提取工艺合理可靠,可为臭椿的开发应用提供实验依据。

OBJECTIVE To optimize ultrasonic-assisted extraction technology of canthin-6-one from bark of Ailanthus altissima.METHODS With yield of canthin-6-one as index,extraction technology parameters were optimized by central composite design-response surface methodology on the basis of the single factor investigation of conditions for extracting canthin-6-one by ethanol.RESULTS Optimum extraction conditions were as follows：ethanol concentration 68%,liquid-solid ratio 32 m L·g^-1,ultrasound temperature 54℃,extraction time 38 min.Under these conditions,yield of canthin-6-one was26.19μg·g^-1,which was close to the predicted value（26.31μg·g^-1）.CONCLUSION This optimized technology is reasonable and reliable to provide a basis for development and application of Ailanthus altissima.