四倍体黄芩及二倍体黄芩遗传差异的ISSR分析

Analysis of Genetic Diversity Between Tetraploid and Diploid Scutellaria Baicalensis by ISSR Markers

目的分析研究四倍体黄芩新品系D20和二倍体黄芩之间的遗传差异。方法采用ISSR分子标记技术对四倍体黄芩及二倍体黄芩之间的遗传差异进行研究。结果从100条引物中筛选得到9条ISSR引物用于正式扩增,多态性百分率为84.2%;遗传距离的变异范围是0.086 9~0.242 9,聚类分析结果将黄芩样本分为两大类,二倍体聚为一类,四倍体聚为另一类。结论四倍体黄芩新品系D20和二倍体黄芩存在明显的遗传差异,ISSR分子标记能有效的揭示材料间的多态性。

OBJECTIVE To study the genetic variation between tetraploid and diploid Scutellaria baicalensis. METHODS ISSR molecular markers were used to study the genetic differences between tetraploid and diploid of Scutellaria baicalensis. RESULTS ISSR primers were screened from 100 primers for formal amplification, the percentage of polymorphism reached 84.2%. The variation range of genetic distance was 0.086 9-0.242 9. The UPGMA clustering tree divided the samples into two major groups. The diploid groups were clustered into one category, and the tetraploid were clustered into another. CONCLUSION There are genetic differences between tetraploid and diploid Scutellaria baicalensis Georgi, and ISSR molecular markers can effectively reveal the polymorphism among materials.