可溶性α淀粉样前体蛋白对蛛网膜下腔出血大鼠神经细胞凋亡及神经功能的影响

Effect of soluble amyloid precursor proteinαon nerve cell apoptosis and neurological function in subarachnoid hemorrhage rats

目的探讨可溶性α淀粉样前体蛋白(solubleαform of amyloid precursor protein,sAPPα)对蛛网膜下腔出血(subarachnoid hemorrhage,SAH)大鼠神经细胞凋亡及神经功能的影响。方法选择雄性SD大鼠60只,随机分为对照组、生理盐水组(SAH+生理盐水)和sAPPα组(SAH+sAPPα),每组20只。大鼠自体血于视交叉前池建立SAH模型,生理盐水组在SAH模型建立后脑室注入生理盐水,sAPPα组在SAH模型建立后脑室注入sAPPα。各组分别于模型建立后取大鼠10只,注药3d后行组织凋亡细胞检测,采用TUNEL法测定凋亡细胞,并进行免疫荧光检测;各组分别于模型建立后取大鼠10只,注药3d后进行神经功能评分。结果对照组大鼠颞叶脑组织仅有很少量阳性凋亡细胞,而生理盐水组脑组织阳性凋亡细胞较对照组明显增多,差异有统计学意义(P<0.05)。与生理盐水组比较,sAPPα组脑组织阳性凋亡细胞明显降低,差异有统计学意义(P<0.05)。对照组、生理盐水组和sAPPα组用药3d后神经功能评分分别为(26.7±0.5)分、(13.9±0.7)分和(23.0±0.8)分。sAPPα组神经功能评分较生理盐水组明显增高,差异有统计学意义(P<0.05)。结论 sAPPα可通过抑制SAH后神经细胞凋亡,减轻继发损伤,从而改善神经功能恢复。

Objective To study the effect of soluble amyloid precursor proteinα（sAPPα）on nerve cell apoptosis and neurological function in subarachnoid hemorrhage（SAH）rats.Methods Sixty male SD rats were randomly divided into control group（n=20）,SAH＋saline group（n=20）and SAH＋sAPPαgroup（n=20）.A SAH model was established by injecting autologous blood into cistern magna in rats.After a SAH model was established for SAH＋saline group and SAH＋sAPPαgroup by injecting saline and sAPPαrespectively into the cistern magna of rats,the apoptotic cells were detected by immunofluorescene with TUNEL staining and the neurological function was scored in 10 rats from each group on day 3 after injection of sAPPαand saline.ResultsThe number of apoptotic cells in brain tissue was significantly greater in SAH＋saline group than in control group（P〈0.05）and was significantly smaller in SAH＋sAPPαgroup than in SAH＋saline group（P〈0.05）.The neurological function score was 26.7±0.5,13.9±0.7 and 23.0±0.8 respectively in control group,SAH＋saline group and SAH＋sAPPαgroup.ConclusionsAPPαalleviates secondary damage of neurological function by inhibiting the apoptosis of nerve cells in rats after SAH and can thus improve their neurological function.