红景天提取物不同纯化工艺的比较

Comparison of Different Purification Processes for the Extract of Herba Rhodiola

目的:比较不同纯化工艺对红景天提取物中红景天苷含量的影响,为其规模化生产提供参考。方法:分别采用乙醇沉淀法、澄清剂沉淀法、AB-8型大孔树脂分离法纯化红景天水提液,并用HPLC法测定纯化后溶液中红景天苷含量,筛选最佳纯化工艺。结果:AB-8型大孔树脂纯化后得到的溶液中红景天苷含量最高达到0.272 5 mg·ml^(-1),乙醇沉淀法次之,其中以70%浓度醇沉效果较好,澄清剂沉淀法红景天苷损失较多。结论:采用AB-8型大孔树脂分离法纯化红景天苷具有得率高,选择性好,可再生等特点,适合红景天苷规模化生产应用。

Objective： To compare the effects of different purification processes on the content of salidroside in the extract of Herba Rhodiola to provide reference for the large-scale production. Methods： Ethanol precipitation,clarifier precipitation and AB-8 macroporous resin separation was respectively used to purify the extract of Herba Rhodiolae,and HPLC was used to determine the content of salidroside to screen the optimum purification process. Results： After the purification by AB-8 macroporous resin,the content of salidroside was as high as 0. 272 5 mg·ml-1 followed by that by ethanol precipitation（ 70% alcohol）,and the clarifier precipitation lost more salidroside. Conclusion： The purification of salidroside by AB-8 macroporous resin separation is with high yield,good selectivity,promising reproducibility and so on,which is suitable for the large-scale production of salidroside.