驴胶补血颗粒对环磷酰胺致小鼠白细胞减少症的作用及代谢组学机制研究

Investigation on positive effect and possible mechanisms of Lvjiao Buxue Granules treatment on cyclophosphamide-induced leucopenia mice through ~1H-NMR based metabolomics approach

目的观察驴胶补血颗粒对环磷酰胺致小鼠白细胞减少症的改善作用,并采用1H-NMR代谢组学方法探讨其作用机制。方法采用ip环磷酰胺的方法制备小鼠白细胞(WBC)减少症模型,测定WBC、红细胞(RBC)、血红蛋白(HGB)和血小板(PLT)等血液常规指标。利用1H-NMR代谢组学技术分析小鼠血清中内源性代谢产物的变化,并对差异代谢产物进行通路分析,探讨驴胶补血颗粒对WBC减少症小鼠血清内源性代谢产物的调节作用及其代谢通路调控机制。结果与对照组比较,模型组小鼠WBC、RBC、HGB以及PLT水平显著降低(P<0.01),而驴胶补血颗粒能显著升高小鼠WBC和PLT水平(P<0.05),并回调潜在的差异代谢产物的水平。结论驴胶补血颗粒可能通过调节机体免疫功能、调控能量代谢和氨基酸代谢达到改善WBC减少症的作用。

Objective To investigate the positive effect and possible mechanism of Lvjiao Buxue Granules on the leukopenia induced by cyclophosphamide in mice using 1 H-NMR metabolomics approach. Methods Cyclophosphamide was used to establish the leucopenia mice, and the content of white blood cells, red blood cells, hemoglobin, platelets and other routine blood indexes were measured. The changes of endogenous metabolites in serum were analyzed by 1 H-NMR metabolomics to investigate the regulation effect of Lvjiao Buxue Granules on serum endogenous metabolites in mice with leukopenia and the regulation mechanism of metabolic pathway. Results Compared with the control group, the contents of white blood cells, red blood cells, hemoglobin, and platelets in the model group were decreased significantly（P〈 0.01）, while the level of white blood cells and platelets in the treatment group were increased significantly（P〈 0.05）, and Lvjiao Buxue Granules can call back the level of potential differences metabolites. Conclusion Lvjiao Buxue Granules can improve leukopenia by regulating the immune function, energy metabolism, and amino acid metabolism.