六黄合剂对胰岛素抵抗大鼠血管内皮PI3-K/Akt信号通路影响

Effect of Liuhuang mixture on PI3-K/Akt signal transduction of endothelial cells in insulin resistant rats

目的了解六黄合剂对胰岛素抵抗大鼠血管内皮保护的作用机制。方法健康SD大鼠,按体重随机分为空白组、模型组、六黄合剂组和罗格列酮组,每组10只。应用高脂饲料联合肌注地塞米松的方法诱导大鼠胰岛素抵抗模型,分别给予罗格列酮和六黄合剂干预,7周后应用RT-PCR法测定大鼠胸主动脉PI3-Km RNA、Aktm RNA和e NOS m RNA的表达水平。结果与模型组比较,六黄合剂组胸主动脉Aktm RNA表达水平(0.20±0.05)明显升高(P<0.05),PI3-Km RNA和e NOS m RNA的表达水平也升高,分别为(1.07±0.31)和(0.87±0.27),差异无统计学意义。结论六黄合剂通过上调PI3-K/Akt信号通路来发挥对胰岛素抵抗大鼠血管内皮的保护作用。

Objective To observe protective effect of Liuhuang mixture （a traditional Chinese herbal compound made of Astragalus, Polygonatum sibiricum, Cattail pollen, Curcuma longa L., Coptis chinensis, and Rheum officinale） on endothelial cells in insulin resistant rats and its mechanism. Methods Forty healthy Sprague-Dawley rats were randomly divided into four groups： normal, model, Liuhuang mixture, and rosiglitazon group based on their body weight （10 in each group）. Insulin resistant rat model was established by feeding high fat food combined with injection of dexamethasone and the rats were interfered with rosiglitazon and Liuhuang mixture. The changes in the mRNA expressions of phosphatidylinositol 3-kinase （PI3-K）, protein kinase B （Akt）, and endothelial nitric oxide synthase （eNOS） in thoracic aorta endothelial cells of the rats were determined with real-time reverse transcription-PCR （RT-PCR） 7 weeks after the intervention. Results The expression of Akt mRNA （0.20±0.05） in thoracic aorta of the rats treated with Liuhuang mixture was higher than that of the rats in model group （P 〈 0.05）. Compared with those of the model group, the expressions of PI3-K mRNA （1.07± 0.31） and eNOS mRNA （0.87 ± 0.27） were increased after the treatment of Liuhuang mixture, but without significance. Conclusion Liuhuang mixture can effectively protect the endothelial cells in insulin resistant rats by increasing PI3-K/Akt signal transduction.