基质金属蛋白酶-2、基质金属蛋白酶组织抑制因子-2在不同年龄阶段成人腹股沟疝患者腹直肌前鞘中的表达及意义

Expression and significance of MMP-2 and TIMP-2 in adults' anterior rectus sheath of patients with inguinal hernia in different age stage

目的探讨基质金属蛋白酶-2(matrix metallopro-teinase-2,MMP-2)、基质金属蛋白酶组织抑制因子-2(tissue inhibitor-2,TIMP-2)在不同年龄阶段成人腹股沟疝患者腹直肌前鞘中的表达及意义。方法选择2016年2月至2016年11月,郑州大学第一附属医院行手术治疗的58例患者,其中试验组腹股沟疝患者48例,按年龄段分为4组,对照组无疝患者10例,均为男性。取材部位均为下腹部腹直肌前鞘,用实时荧光定量PCR技术检测成人不同年龄阶段组织标本中MMP-2mRNA及TIMP-2 mRNA的表达水平。结果 MMP-2mRNA表达:对照组1.530±0.612,试验1组1.561±0.222,试验2组2.305±0.213,试验3组2.918±0.392,试验4组3.270±0.259。4个试验组与对照组比较,均呈高表达,试验3组及4组分别与对照组比较,差异均有统计学意义(P<0.05),试验1组及2组分别与对照组比较,差异均无统计学意义(P>0.05)。4个试验组相互比较,随着年龄增大表达逐渐增高,1组与3组比较、1组与4组比较、2组与4组比较差异均有统计学意义(P<0.05),其余组间比较差异均无统计学意义(P>0.05)。TIMP-2 mRNA表达:对照组1.021±0.395,试验1组0.996±0.096,试验2组0.813±0.049,试验3组0.504±0.035,试验4组0.374±0.026。4个试验组与对照组比较,均呈低表达,试验3组及4组分别与对照组比较差异均有统计学意义(P<0.05),试验1组及2组分别与对照组比较差异均无统计学意义(P>0.05)。4个试验组相互比较,随着年龄增大表达逐渐降低,1组与3组比较、1组与4组比较、2组与4组比较差异均有统计学意义(P<0.05),其余组间比较差异均无统计学意义(P>0.05)。4个试验组均值:MMP-2 mRNA为2.513±1.135、TIMP-2 mRNA为0.672±0.313,与对照组1.530±0.612、1.021±0.395比较,差异均有统计学意义(P<0.05)。结论成人腹股沟疝患者腹直肌前鞘中,MMP-2mRNA呈高表达,TIMP-2mRNA呈低表达,与无疝者比较有显著差异,表明2者与腹股沟疝的发生密切相关。随着年龄增大,MMP-2mRNA逐渐增高,TIMP-2mRNA逐渐降低,70岁以上表达显著,这是老年人随着年龄增大腹股沟疝患病率增高的重要原因之一。

Objective To investigate the association of MMP-2 and TIMP-2 in adults＇ anterior rectus sheath of patients with inguinal hernia in different age stage. Methods There were fifty-eight patients undergoing surgical treatment in the First Affiliated Hospital of Zhengzhou University from February 2016 to November 2016, including forty-eight cases of inguinal hernia in the experimental group and ten cases of non-hernia in the control group, all of whom male. The expression levels of MMP-2 mRNA and TIMP-2 mRNA of the cases at different age were detected by Real-Time PCR. Results The expression of MMP-2 mRNA： the control group was 1.530±0.612, the group 1 was 1.561±0.222, the group 2 was 2.305±0.213, the group 3 was 2.918±0.392, the group 4 was 3.270±0.259. The four experimental groups showed high expression compared with the control group. The differences were significant comparing the group 3 and the group 4 with the control one, and there was no statistical significance to compare the group 1 and the group 2 with the control one. When compared with each other between the 4 groups, the expression gradually increased with age. The expression of TIMP-2 mRNA： the control group was 1.021±0.395, the group 1 was 0.996±0.096, the group 2 was 0.813±0.049, the group 3 was 0.504±0.035, the group 4 was 0.374±0.026. The four experimental groups showed low expression comparing with the control group. The differences were significant comparing the group 3 and the group 4 with the control one, and there was no statistical significance to compare the group 1 and the group 2 with the control one. When compared with each other between the 4 groups, the expression gradually decreased with age. The average value of MMP-2 mRNA and TIMP-2 mRNA of 4 groups were significantly different with the control group, respectively. Conclusion The expression of MMP-2 mRNA in the anterior sheath of the abdominal rectus abdominis in adult inguinal hernia was high, and the low expression of TIMP-2 mRNA was significantly different from that of the non-hernia, indicating that the two were closely related to the occurrence of inguinal hernia. As the age increase, MMP-2 mRNA gradually increase, and TIMP-2 mRNA gradually decreases. This is one of the important reasons for the increase of the prevalence of inguinal hernia with age.