黄皮果果核挥发油抗UV致小鼠皮肤光老化损伤的实验研究

Study on the effect of essential oil from fruit kernel of Wampee against UV-induced skin photoaging in mice

通过水蒸气蒸馏法和冷冻结晶法对黄皮果果核挥发油进行提取和分离,将所得黄皮果果核挥发油涂于去毛KM小鼠背部,再进行UVA/UVB联合照射,从皮肤表观评价得分及HE染色结果对其抗光老化作用进行评价。结果表明,1.9%的维生素E供试液作为阳性对照药,能起到显著的抗皮肤光老化作用,2.5%,5%和10%的黄皮果果核挥发油供试液可起到抗UV所致真皮病变的作用,5%和10%时可改善UV照射所引起的表皮增厚及皱褶产生等表皮病变。

The essential oil from fruit kernel of Wampee was extracted by steam distillation and separated by frozen crystallization. Then the essential oil was applied to the back of hairless KM mice. Score of skin clinical observation and histopathological examination were performed to evaluate the anti-photoaging effects after UVA/UVB combined irradiation. Results show that 1. 9% Vitamin E as the positive control plays a significant role in anti-photoaging,The essential oil from fruit kernel of Wampee at the dose of 2. 5%,5% and 10% can play a role in resisting UV-induced dermis lesions,and can improve UV-induced epidermal lesions like epidermal thickening and wrinkle formation at 5% and 10%.