三氧化二砷对急性髓系白血病细胞HL-60 Cdc20及Mad2的影响

Effects of Arsenic Trioxide on Cdc20 and Mad2 in Acute Myeloid Leukemia HL-60 Cell Line

目的:探讨三氧化二砷(As_2O_3)对急性髓系白血病细胞HL-60增殖过程中Cdc20及Mad2的影响。方法:不同浓度As_2O_3处理HL-60细胞24、48、72 h后CCK-8法检测细胞增殖情况;Real-Time PCR及Western blot技术检测不同浓度As_2O_3作用于HL-60细胞48 h后Mad2和Cdc20 mRNA及蛋白表达情况。结果:As_2O_3能显著抑制HL-60细胞增殖,并具有良好的时间剂量相关性。RT-PCR和Western blot检测结果表明,As_2O_34、8、10μmol/L作用于HL-60细胞后,Mad2表达上调,Cdc20表达下降(P<0.05)。结论:As_2O_3对人急性髓系白血病HL-60细胞增殖具有较明显的抑制作用,其机制可能与上调Mad2表达和下调Cdc20表达有关。

Objective： To investigate the effects of arsenic trioxide（ As2O3） on Cdc20 and Mad2 in process of AML HL-60 cell proliferation.Methods： The proliferation of HL-60 cells was detected by CCK-8 method at different concentrations of arsenic trioxide for 24,48 and 72 hours.The cell morphological changes were observed by inverted microscopy.The expressions of Mad2 and Cdc20 mRNA and protein in HL-60 cells treated with As2O3 for 48 h were detected by real-time PCR and Western blot respectively.Results： Arsenic trioxide significantly inhibited the HL-60 cell proliferation and displayed a good time-dose correlation.RT-PCR and Western blot showed that the expression of Mad2 was up-regulated and the expression of Cdc20 was down-regulated in HL-60 cells treated with arsenic trioxide of different concentration（4,8,10 μmol/L）.Conclusion： Arsenic trioxide can inhibit the human acute myeloid leukemia HL-60 cell proliferation,and its mechanism may be related with up-regulation of Mad2 expression and down-regulation of Cdc20 expression.