罕见B(A)亚型新生儿溶血病及血型鉴定

The hemolytic disease of a rare B(A) subtype newborn and its blood typing

目的对罕见B（A）亚型新生儿溶血病进行血清学分析,并探讨该类血型的鉴定策略。方法对1例新生儿溶血病标本应用血型血清学方法进行新生儿溶血病相关检测,对发现的B（A）亚型应用血型血清学、家系调查、分子生物学等方法进行鉴定。结果本例为罕见B（A）亚型新生儿溶血病,并仅与抗原性相对较弱的A抗原相关。家系调查发现3例B（A）亚型,基因分型及序列测定证实为B（A）07型。结论罕见B（A）亚型新生儿溶血病具有重要临床意义,其血型鉴定过程需要采用多种方法。

Objective To analyze the serological properties of The hemolytic disease of a rare B（A） subtype newborn and to discuss its blood typing strategy.Methods Serological tests were conducted to characterize the hemolytic disease condition of a newborn infant. Blood typing was performed by a combination of serology tests,family history surveys,molecular biology tests,etc to further confirm a rare B（A） subtype. Results The tests concluded that the infant suffered a rare B（A） subtype hemolytic disease,which is specifically associated with the relatively weak antigenicity of antigen A. We found 3 family members with B（A） subtype by the family history surveys. Genotyping and sequencing confirmed that all of these members were haplotype B（A） 07. Conclusion This case of rare B（A） subtype hemolytic disease presents important clinical significance. The identification of such a subtype requires a variety of methods.