HPLC法快速测定植物油中视黄醇和生育酚

Rapid determination of retinol and tocopherol in vegetable oils by high performance liquid chromatography

建立一种同时快速测定植物油中天然视黄醇和生育酚含量的检测方法。植物油样品经丙酮:甲醇(v/v=70:30)溶解定容提取后,以C18为色谱柱,甲醇:水(v/v=98:2)为流动相,对所提试样中视黄醇、α-生育酚、δ-生育酚和γ-生育酚含量进行高效液相色谱检测。实验结果表明,在325nm波长下,视黄醇在(0.102~2.04)μg/mL范围内呈良好线性关系;在290 nm波长下,α-生育酚、δ-生育酚、γ-生育酚别在(0.9885~19.77)μg/mL、(2.0845~41.69)μg/mL、(3.024~63.22)μg/mL范围内呈良好线性关系,相关系数均在0.9995以上,方法检测限(0.1~3.0)mg/kg;用火麻油作添加回收率和精密度实验,回收率在85.0%~105.0%之间,变异系数不超过5%。该方法具有简便、灵敏、可靠等优点,可满足植物油中视黄醇和生育酚含量的同时批量快速检测。

To determine of retinol and tocopherol in vegetable oils samples by high performance liquid chromatography（HPLC）. The sample was pretreated with acetone：methanol（v/v=70：30） directly, and then separated by C18 chromatographic column. The mobile phase consisted of methanol 98% and water 2%. The analytes were detected by high performance liquid chromatography（HPLC）. By the 325 nm wavelength, the linear ranges of retinol was from 0.102 μg/mL to 2.04 μg/mL and by the 290 nm wavelength, the linear ranges of alpha tocopherol, delta tocopherol, and gamma tocopherol were from 0.9885 μg/mL to 19.77 μg/mL, 2.0845 μg/mL to 41.69 μg/mL, and 3.024 μg/mL to 63.22 μg/mL with the correlation（r） above 0.9995. The minimum detection limits were（0.1~3.0）mg/kg. The recoveries were in range of 85.0% to 105.0% with the relative standard deviations（RSD） less than 5% in heat sesame oils. This method is simple, accurate, reliable and suitable for the simultaneous determination of retinol and tocopherol in vegetable oils.