人参皂苷Rb1通过抑制炎症反应及氧化应激改善老年小鼠内皮功能

Ginsenoside Rb1 Improves Endothelial Function via Inhibiton of Inflammation and oxidative stress in Aged Mice

目的探讨人参皂苷Rb1对老年鼠主动脉内皮功能的影响及其可能机制。方法 10只雌性C57BL/6小鼠为年轻对照组(3月);将30只雌性老年鼠(22月)随机分为老年对照组,老年Rb1低剂量组(10mg/kg Rb1)和老年Rb1高剂量组(20mg/kg Rb1)。分别给予相应处理6周,验证人参皂苷Rb1对老年鼠主动脉内皮炎症指标及氧化应激的作用。结果与年轻鼠相比,老年鼠体内炎症指标单核细胞趋化蛋白-1(MCP-1)、纤溶酶原激活物抑制因子1(PAI-1)、细胞黏附分子-1(ICAM-1)和丙二醛(MDA)表达均显著增加;而超氧化物歧化酶1(SOD1)、内皮型一氧化氮合酶(eNOS)和一氧化氮(NO)表达明显减少。给予不同剂量Rb1后,前述指标表达均得到一定程度的逆转。结论人参皂苷Rb1通过抑制老年鼠体内炎症反应和氧化应激,从而发挥改善主动脉内皮功能的作用。

Objective To explore the effect of ginsenoside Rb1（Rb1）on the endothelial function in aged mice and the possibly involved mechanisms.Methods 40 female mice were divided to young control group,aged control group,aged L-Rb1 group and aged H-Rb1 group.The mice were intraperitoneally injected with Rb1 or normal saline respectively for 6 weeks.And then several parameters were detected to demonstrate the effect of Rb1 on inflammation and oxidative stress.Results Compared to young control mice,there were more expression of MCP-1,PAI-1,ICAM-1 and MDA,while less expression of SOD1,eNOS and NO.Rb1 treatment was able to reverse the expression profile of parameters mentioned above.Conclusion Ginsenoside Rb1 Improves Endothelial Function via Inhibiton of Inflammation and oxidative stress in Aged Mice.