ALC1蛋白在食管鳞癌中的表达及其对细胞增殖侵袭迁移的影响

Expression of ALC1 in tissues of esophageal squamous cell carcinoma and its effect on ESCC cell proliferation, invasion, and migration

目的:探讨ALC1(amplified in liver cancer 1)在食管鳞癌组织中的表达及与临床病理特征及总生存率关系,检测过表达ALC1基因对食管癌细胞恶性生物学行为的影响。方法:采用免疫组织化学方法检测245例食管鳞癌组织及癌旁组织中ALC1蛋白的表达,并探讨其与食管鳞癌患者性别、年龄、分化程度、浸润深度、TNM分期、远处淋巴结转移关系及总生存率关系;采用MTT法、克隆形成实验、Transwell实验及细胞划痕实验等观察高表达ALC1基因在食管癌细胞中的增殖、侵袭及迁移作用。结果:ALC1蛋白在食管癌组织中的阳性表达率明显高于癌旁组织(41.6%vs.21.2%,P<0.05);ALC1的高表达与肿瘤的浸润深度、TNM分期和淋巴结转移明显相关(P<0.05)。ALC1高表达的食管鳞癌患者总生存率低。ALC1基因能够促进KYSE30食管癌细胞过度增殖、侵袭和迁移。结论:ALC1表达升高可能与食管鳞癌的发生、发展相关,导致总生存率下降,高表达的ALC1基因增强KYSE30食管癌细胞的增殖、侵袭及迁移能力,检测ALC1可能为食管癌预后判断提供依据。

Objective： To investigate the expression of ALC1 protein during esophageal squamous cell carcinoma（ESCC） development and progression, so as to explore its association with clinicopathological characteristics and overall survival of ESCC patients, and the effect of ALC1 overexpression on malignant biological behavior of esophageal squamous cells. Methods： Immunohistochemistry（IHC）was used to detect ALC1 protein expression in 245 primary ESCC tissues and their paired normal esophageal mucous membranes, and to determine its correlation to gender, age, tumor cell differentiation, invasion, TNM stage, lymph nodes metastasis, and overall survival rate of ESCC patients. MTT assay, colony formation assay, transwell invasion, and wound healing assay were used to observe the effect of ALC1 on ESCC cell proliferation, invasion, and migration. Results： The expression ratio of ALC1 in esophageal squamous cell carcinoma was higher compared with that in their paired normal esophageal mucous membranes（41.6% vs. 21.2%, P〈0.05）. Upregulation of ALC1 was associated with ESCC invasion, TNM stage, and lymph node metastasis（P〈0.05）. The overall survival of ESCC patients with ALC1 overexpression was significantly lower than that in patients with downregulated ALC1 expression（P=0.002）. Therefore,ALC1 may promote the proliferation, invasion, and migration of ESCC cells. Conclusions： ALC1 upregulation may play an important role in the progression and development of ESCC. Upregulation of ALC1 leads to poorer disease prognosis, and could promote the proliferation, invasion, and migration of the KYSE30 ESCC cells. Therefore, ALC1 may have potential prognostic value for ESCC patients.