人参皂苷Rg3通过PI3K/AKT信号系统调控CaM基因表达促进胃癌BGC-823细胞的凋亡

Ginsenoside Rg3 regulates CaM gene expression through PI3K/AKT signal system to promote apoptosis of gastric cancer BGC-823 cell

目的:探讨人参皂甙Rg3通过PI3K/AKT信号通路干扰Ca M基因的表达及对胃癌BGC-823细胞凋亡的影响。方法:胃癌BGC-823细胞的培养与传代完成后,Western blotting检测胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)和/或Rg3分别作用胃癌BGC-823细胞后p-AKT和Ca M蛋白表达水平,MTT法检测IGF-1和/或Rg3对胃癌BGC-823细胞增殖的影响,Transwell法检测IGF-1和/或Rg3对胃癌BGC-823细胞侵袭的影响,流式细胞术检测IGF-1和/或Rg3对胃癌BGC-823细胞凋亡的影响。结果:随着IGF-1作用时间延长,胃癌BGC-823细胞中p-AKT蛋白和Ca M蛋白的表达水平均明显提高(均P<0.05);与空白对照组比较,Rg3组明显抑制胃癌BGC-823细胞增殖,而IGF-1组和IGF-1+Rg3组明显促进胃癌BGC-823细胞增殖(均P<0.05);与空白对照组比较,Rg3组明显降低胃癌BGC-823细胞侵袭,而IGF-1组和IGF-1+Rg3组明显促进胃癌BGC-823细胞侵袭能力(均P<0.05);流式细胞术检测显示,与空白对照组比较,Rg3组明显促进胃癌BGC-823细胞凋亡,而IGF-1组和IGF-1+Rg3组明显抑制胃癌BGC-823细胞凋亡(均P<0.05)。结论:人参皂甙Rg3通过阻断PI3K/AKT信号通路来抑制Ca M的表达,进而促进胃癌BGC-823细胞的凋亡。

Objective： To investigate Ginsenoside Rg3 interfering the expression of Ca M through PI3 K/AKT signaling pathway to affect the biological activity of gastric cancer BGC-823 cells. Methods： After the culture and passage of gastric cancer BGC-823 cells,Western blotting was used to detect the expression of p-AKT and Ca M protein in gastric cancer BGC-823 cells treated with IGF-1 and/or Rg3; The effect of IGF-1 and/or Rg3 on the proliferation of BGC-823 cells was detected by MTT assay; The effect of IGF-1 and/or Rg3 on the invasion of BGC-823 cells was detected by Transwell assay; Effect of IGF-1 and/or Rg3 on apoptosis of BGC-823 cells was detected by Flow Cytometry. Results： Western blotting results showed that the expression of p-AKT and Ca M protein increased in BGC-823 cells with the prolongation of IGF-1 treatment（all P〈0.05）; Compared with the blank control group, Rg3 significantly inhibited the proliferation of BGC-823 cells, while IGF-1 and IGF-1＋Rg3 significantly promoted the cell proliferation（all P〈0.05）; Compared with the blank control group, Rg3 significantly reduced the invasion of BGC-823 cells, while IGF-1 and IGF-1＋Rg3 significantly promoted the invasion of BGC-823 cells（all P〈0.05）;Flow cytometry showed that compared with the blank control group, Rg3 significantly promoted the apoptosis of BGC-823 cells, while IGF-1 and IGF-1＋Rg3 significantly inhibited the apoptosis of BGC-823 cells（all P〈0.05）. Conclusion： Ginsenoside Rg3 inhibits the expression of Ca M by blocking PI3 K/AKT signaling pathway, thereby promoting the apoptosis of gastric cancer BGC-823 cells.