转BpCUCt白桦生长特征及内源IAA含量的变化

Growth Characteristics and Endogenous IAA Content of BpCUCt Transgenic Lines in Betula platyphylla

为了揭示白桦Bp CUCt基因的功能,分别构建了Bp CUC2启动子及35S启动子驱动的Bp CUCt载体,以白桦合子胚为受体开展转基因工作,观察转基因株系表型变化,并测定转基因株系和WT的内源激素IAA含量。结果表明,对比转基因株系生长特性可知,转Bp CUCt基因白桦株系比WT矮小,生长也比较缓慢,转基因株系节间距均变长,而Pro CUC2::Bp CUCt转基因株系叶片边缘比WT叶片平滑,35S::Bp CUCt转基因株系叶边缘与野生型并无明显区别。由转基因白桦和WT的内源激素IAA含量可知,83.33%的转基因株系左侧叶片、右侧叶片及主脉IAA含量均显著高于WT株系。表明Bp CUCt基因在一定程度上与Bp CUC2基因表现出相同的功能,同时改变白桦内源生长素IAA的含量。

To identify the function of BpCUCt in birch, we constructed two vectors that could be expressed in plants, namely ProCUC2：： BpCUCt and 35S：：BpCUCt. Meanwhile, we regarded zygotic embryo from elite birch trees as transgenic receptor, observed the changed phenotypes of transgenic lines and measured the endogenous IAA content. Compared to the wild type, the BpCUCt transgenic lines were with smaller height, lower growth characters and longer knot distances. However, the leaf margin of ProCUC2： ：BpCUCt lines were smoother than the wild type, transgenic lines of 35S： ：BpCUCt did not distinguish from the wild type. In view of the endogenous IAA content from the transgenic lines and wild type, the 83.33% of transgenic lines that was with more endogenous IAA content than wild type in the left leaf, the right leaf and the main vein. In conclusion, to some degree, the function of BpCUCt played similar roles in the function of BpCUC2, meanwhile, this gene was also related to endogenous IAA content in birch trees.