匀浆法提取沙枣果总黄酮工艺研究

Homogenate Extraction Process in Total Flavonoids from Elaeagnus angustifolia Fruit

沙枣常用于治疗脾胃虚弱、消化不良、肠炎腹泻、肺热咳嗽等疾病。为更好的开发利用沙枣植物资源,本研究通过传统水浴提取法和匀浆提取法的单因素对比实验,确定了匀浆法提取沙枣果总黄酮的提取方案。通过正交优化,获得匀浆法最优提取工艺为:乙醇浓度55%、料液比1∶30 g·m L-1、匀浆时间5 min。在优化工艺条件下,沙枣果总黄酮的得率为6.57%。本研究为沙枣果的综合开发利用提供理论依据。

Elaeagnus angustifolia is usually used in the treatment of spleen and stomach, indigestion, enteritis, diarrhea, cough and other diseases. In this study, the homogenate extraction method and the traditional method were compared by single factor experiment, and the method of homogenate extraction total flavonoids from E. angustifolia fruit was determined. The optimum technology conditions were as follows： ethanol concentration was 55%, solid-liquid ratio was 1：30 g · mL-1 and the extraction time was 5 rain. Under the optimal conditions, the total flavonoids of E. angustifolia fruit yield rate was 6.57%. This study would provide a theoretical basis for the comprehensive development and utilization of E. angustifolia fruit.